Article (Scientific journals)
Structural determinants required to target penicillin-binding protein 3 to the septum of Escherichia coli
Piette, André; Fraipont, Claudine; den Blaauwen, Tanneke et al.
2004In Journal of Bacteriology, 186 (18), p. 6110-6117
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Keywords :
PBP3 ,divisome
Abstract :
[en] In Escherichia coli, cell division is mediated by the concerted action of about 12 proteins that assemble at the division site to presumably form a complex called the divisome. Among these essential division proteins, the multimodular class B penicillin-binding protein 3 (PBP3), which is specifically involved in septall peptidoglycan synthesis, consists of a short intraceflular M1-R23 peptide fused to a F24-L39 membrane anchor that is linked via a G40-S70 peptide to an R71-1236 noncatalytic module itself linked to a D237-V577 catalytic penicillin -binding module. On the basis of localization analyses of PBP3 mutants fused to green fluorescent protein by fluorescence microscopy, it appears that the first 56 amino acid residues of PBP3 containing the membrane anchor and the G40-E56 peptide contain the structural determinants required to target the protein to the cell division site and that none of the putative protein interaction sites present in the noncatalytic module are essential for the positioning of the protein to the division site. Based on the effects of increasing production of FtsQ or FtsW on the division of cells expressing PBP3 mutants, it is suggested that these proteins could interact. We postulate that FtsQ could play a role in regulating the assembly of these division proteins at the division site and the activity of the peptidoglycan assembly machineries within the divisome.
Disciplines :
Biochemistry, biophysics & molecular biology
Microbiology
Author, co-author :
Piette, André ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Fraipont, Claudine ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
den Blaauwen, Tanneke;  University of Amsterdam > Swammerdam Institute for Life Sciences
Aarsman, Mirjam E. G.;  University of Amsterdam > Swammerdam Institute for Life Sciences
Pastoret, Soumya;  Université de Liège - ULiège > Centre d'Ingénierie des protéines
Nguyen-Distèche, Martine ;  Université de Liège - ULiège > Centre d'Ingénierie des protéines
Language :
English
Title :
Structural determinants required to target penicillin-binding protein 3 to the septum of Escherichia coli
Publication date :
September 2004
Journal title :
Journal of Bacteriology
ISSN :
0021-9193
eISSN :
1098-5530
Publisher :
Amer Soc Microbiology, Washington, United States - Washington
Volume :
186
Issue :
18
Pages :
6110-6117
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 June 2009

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