Reference : Functional analysis of the cell division protein FtsW of Escherichia coli
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/15104
Functional analysis of the cell division protein FtsW of Escherichia coli
English
Pastoret, Soumya [Université de Liège - ULg > > Centre d'ingénierie des protéines > > >]
Fraipont, Claudine mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
den Blaauwen, Tanneke mailto [>Swammerdam Institute for Life Sciences > > > > > >]
Wolf, Benoît mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Aarsman, Mirjam E. G. [Swammerdam Institute for Life Sciences > > >Molecular Cytology > > >]
Piette, André mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Thomas, Annick [Faculté Universitaire des Sciences Agronomiques de Gembloux > Centre de Biophysique Moléculaire Numérique > > >]
Brasseur, Robert mailto [Faculté Universitaire des Sciences Agronomiques de Gembloux - FUSAGx > Centre de Biophysique Moléculaire Numérique > > >]
Nguyen-Distèche, Martine mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines > > >]
Dec-2004
Journal of Bacteriology
American Society for Microbiology (ASM)
186
24
8370-8379
Yes (verified by ORBi)
International
0021-9193
1098-5530
Washington
DC
[en] Divisome ; FtsW
[en] Site-directed mutagenesis experiments combined with fluorescence microscopy shed light on the role of Escherichia coli FtsW, a membrane protein belonging to the SEDS family that is involved in peptidoglycan assembly during cell elongation, division, and sporulation. This essential cell division protein has 10 transmembrane segments (TMSs). It is a late recruit to the division site and is required for subsequent recruitment of penicillin-binding protein 3 (PBP3) catalyzing peptide cross-linking. The results allow identification of several domains of the protein with distinct functions. The localization of PBP3 to the septum was found to be dependent on the periplasmic loop located between TMSs 9 and 10. The E240-A249 amphiphilic peptide in the periplasmic loop between TMSs 7 and 8 appears to be a key element in the functioning of FtsW in the septal peptidoglycan assembly machineries. The intracellular loop (containing the R166-FI78 amphiphilic peptide) between TMSs 4 and 5 and Gly 311 in TMS 8 are important components of the amino acid sequence-folding information.
Researchers
http://hdl.handle.net/2268/15104
10.1128/JB.186.24.8370-8379.2004

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