Article (Scientific journals)
Functional analysis of the cell division protein FtsW of Escherichia coli
Pastoret, Soumya; Fraipont, Claudine; den Blaauwen, Tanneke et al.
2004In Journal of Bacteriology, 186 (24), p. 8370-8379
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Keywords :
Divisome; FtsW
Abstract :
[en] Site-directed mutagenesis experiments combined with fluorescence microscopy shed light on the role of Escherichia coli FtsW, a membrane protein belonging to the SEDS family that is involved in peptidoglycan assembly during cell elongation, division, and sporulation. This essential cell division protein has 10 transmembrane segments (TMSs). It is a late recruit to the division site and is required for subsequent recruitment of penicillin-binding protein 3 (PBP3) catalyzing peptide cross-linking. The results allow identification of several domains of the protein with distinct functions. The localization of PBP3 to the septum was found to be dependent on the periplasmic loop located between TMSs 9 and 10. The E240-A249 amphiphilic peptide in the periplasmic loop between TMSs 7 and 8 appears to be a key element in the functioning of FtsW in the septal peptidoglycan assembly machineries. The intracellular loop (containing the R166-FI78 amphiphilic peptide) between TMSs 4 and 5 and Gly 311 in TMS 8 are important components of the amino acid sequence-folding information.
Disciplines :
Microbiology
Author, co-author :
Pastoret, Soumya;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Fraipont, Claudine ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
den Blaauwen, Tanneke;  Swammerdam Institute for Life Sciences
Wolf, Benoît ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Aarsman, Mirjam E. G.;  Swammerdam Institute for Life Sciences > Molecular Cytology
Piette, André ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Thomas, Annick;  Faculté Universitaire des Sciences Agronomiques de Gembloux > Centre de Biophysique Moléculaire Numérique
Brasseur, Robert ;  Faculté Universitaire des Sciences Agronomiques de Gembloux - FUSAGx > Centre de Biophysique Moléculaire Numérique
Nguyen-Distèche, Martine ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Language :
English
Title :
Functional analysis of the cell division protein FtsW of Escherichia coli
Publication date :
December 2004
Journal title :
Journal of Bacteriology
ISSN :
0021-9193
eISSN :
1098-5530
Publisher :
American Society for Microbiology (ASM), Washington, United States - District of Columbia
Volume :
186
Issue :
24
Pages :
8370-8379
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 June 2009

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