Article (Scientific journals)
Interaction between two murein (peptidoglycan) synthases, PBP3 and PBP1B, in Escherichia coli
Bertsche, Ute; Kast, Thomas; Wolf, Benoît et al.
2006In Molecular Microbiology, 61 (3), p. 675-690
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Keywords :
divisome; PBP
Abstract :
[en] The murein (peptidoglycan) sacculus is an essential polymer embedded in the bacterial envelope. The Escherichia coli class B penicillin-binding protein (PBP) 3 is a murein transpeptidase and essential for cell division. In an affinity chromatography experiment, the bifunctional transglycosylase-transpeptidase murein synthase PBP1B was retained by PBP3-sepharose when a membrane fraction of E. coli was applied. The direct protein-protein interaction between purified PBP3 and PBP1B was characterized in vitro by surface plasmon resonance. The interaction was confirmed in vivo employing two different methods: by a bacterial two-hybrid system, and by cross-linking/co-immunoprecipitation. In the bacterial two-hybrid system, a truncated PBP3 comprising the N-terminal 56 amino acids interacted with PBP1B. Both synthases could be cross-linked in vivo in wild-type cells and in cells lacking FtsW or FtsN. PBP1B localized diffusely and in foci at the septation site and also at the side wall. Statistical analysis of the immunofluorescence signals revealed that the localization of PBP1B at the septation site depended on the physical presence of PBP3, but not on the activity of PBP3. These studies have demonstrated, for the first time, a direct interaction between a class B PBP (PBP3) and a class A PBP (PBP1B) in vitro and in vivo, indicating that different murein synthases might act in concert to enlarge the murein sacculus during cell division.
Disciplines :
Microbiology
Biochemistry, biophysics & molecular biology
Author, co-author :
Bertsche, Ute;  Universität Tübingen > Microbielle Genetik
Kast, Thomas;  Max-Planck-Institut für Entwicklungsbiologie > Abteilung Biochemie
Wolf, Benoît ;  Université de Liège - ULiège > Centre d'Ingénierie des protéines
Fraipont, Claudine ;  Université de Liège - ULiège > Centre d'Ingénierie des protéines
Aarsman, Mirjam E. G.;  Swammerdam Institute for Life Sciences > Molecular Cytology
Kannenberg, Kai;  Universität Tübingen > Microbielle Genetik
von Rechenberg, Moritz;  Max-Planck-Institut für Entwicklungsbiologie > Abteilung Biochemie
Nguyen-Distèche, Martine ;  Université de Liège - ULiège > Centre d'Ingénierie des protéines
den Blaauwen, Tanneke;  Swammerdam Institute for Life Sciences > Molecular Cytology
Holtje, Joachim V;  Max-Planck-Institut für Entwicklungsbiologie > Abteilung Biochemie
Vollmer, Waldemar;  Universität Tübingen > Microbielle Genetik
Language :
English
Title :
Interaction between two murein (peptidoglycan) synthases, PBP3 and PBP1B, in Escherichia coli
Publication date :
August 2006
Journal title :
Molecular Microbiology
ISSN :
0950-382X
eISSN :
1365-2958
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
61
Issue :
3
Pages :
675-690
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 June 2009

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