Function of the Chloroplast Hydrogenase in the Microalga Chlamydomonas: The Role of Hydrogenase and State Transitions during Photosynthetic Activation in Anaerobiosis
[en] Like a majority of photosynthetic microorganisms, the green unicellular alga Chlamydomonas reinhardtii may encounter O2 deprived conditions on a regular basis. In response to anaerobiosis or in a respiration defective context, the photosynthetic electron transport chain of Chlamydomonas is remodeled by a state transition process to a conformation that favours the photoproduction of ATP at the expense of reductant synthesis. In some unicellular green algae including Chlamydomonas, anoxia also triggers the induction of a chloroplast-located, oxygen sensitive hydrogenase, which accepts electrons from reduced ferredoxin to convert protons into molecular hydrogen. Although microalgal hydrogen evolution has received
much interest for its biotechnological potential, its physiological role remains unclear. By using specific Chlamydomonas mutants, we demonstrate that the state transition ability and the hydrogenase function are both critical for induction of photosynthesis in anoxia. These two processes are thus important for survival of the cells when they are transiently placed
in an anaerobic environment.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Ghysels, Bart ; Université de Liège - ULiège > Labo de Bioénergétique
Godaux, Damien ; Université de Liège - ULiège > Département des sciences de la vie > Génétique
Matagne, René-Fernand ; Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Cardol, Pierre ; Université de Liège - ULiège > Département des sciences de la vie > Génétique
Franck, Fabrice ; Université de Liège - ULiège > Labo de Bioénergétique
Language :
English
Title :
Function of the Chloroplast Hydrogenase in the Microalga Chlamydomonas: The Role of Hydrogenase and State Transitions during Photosynthetic Activation in Anaerobiosis
Publication date :
23 May 2013
Journal title :
PLoS ONE
eISSN :
1932-6203
Publisher :
Public Library of Science, San Franscisco, United States - California
Volume :
8
Issue :
5
Pages :
e64161
Peer reviewed :
Peer Reviewed verified by ORBi
European Projects :
FP7 - 245070 - SUNBIOPATH - Towards a better sunlight to biomass conversion efficiency in microalgae
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] CE - Commission Européenne [BE]
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