[en] Symbiotic bacteria are known to play important roles in the biology of insects, but
the current knowledge of bacterial communities associated with mosquitoes is very
limited and consequently their contribution to host behaviors is mostly unknown.
In this study, we explored the composition and diversity of mosquito-associated
bacteria in relation with mosquitoes’ habitats. Wild Aedes albopictus and Aedes
aegypti were collected in three different geographic regions of Madagascar.
Culturing methods and denaturing gradient gel electrophoresis (DGGE) and
sequencing of the rrs amplicons revealed that Proteobacteria and Firmicutes were
the major phyla. Isolated bacterial genera were dominated by Bacillus, followed by
Acinetobacter, Agrobacterium and Enterobacter. Common DGGE bands belonged to
Acinetobacter, Asaia, Delftia, Pseudomonas, Enterobacteriaceae and an uncultured
Gammaproteobacterium. Double infection by maternally inherited Wolbachia
pipientis prevailed in 98% of males (n = 272) and 99% of females (n = 413); few
individuals were found to be monoinfected withWolbachia wAlbB strain. Bacterial
diversity (Shannon–Weaver and Simpson indices) differed significantly per habitat
whereas evenness (Pielou index) was similar. Overall, the bacterial composition
and diversity were influenced both by the sex of individuals and by the environment
inhabited by the mosquitoes; the latter might be related to both the
vegetation and the animal host populations that Aedes used as food sources.
Research center :
Université Claude Bernard Lyon 1
Disciplines :
Microbiology Entomology & pest control
Author, co-author :
Zouache, Karima; Université Claude Bernard - Lyon 1 - UCLB > Ecologie Microbienne UMR 5557 > 2011
Raharimalala, Fara Nantenaina ; Université d'Antananarivo - Université Claude Bernard Lyon 1 > Sciences Naturelles > Entomologie - Ecologie Microbienne UMR 5557 > 2011
Raquin, Vincent; Université Claude Bernard - Lyon 1 - UCLB > Ecologie Microbienne UMR 5557 > 2011
Van, Tran Van; Université Claude Bernard - Lyon 1 - UCLB > Ecologie Microbienne UMR 5557 > 2011
Ravelonandro, Pierre; Centre National de Recherche de l'Environnement (CNRE) > Entomologie > 2011
Mavingui, Patrick; Université Claude Bernard - Lyon 1 - UCLB > Ecologie Microbienne UMR 5557 > 2011
Language :
English
Title :
Bacterial diversity of field-caught mosquitoes, Aedes albopictus and Aedes aegypti, from different geographic regions of Madagascar.
Publication date :
2011
Journal title :
FEMS Microbiology Ecology
ISSN :
0168-6496
eISSN :
1574-6941
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Pages :
377–389
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
‘Arthropod Symbioses: from fundamental to pest disease management’.
Funders :
ANR-06-SEST07 FRB-CD-AOOI-07-012 Biodiversité et Développement Durable à Madagascar and COSTaction F0701 ‘Arthropod Symbioses: from fundamental to pest disease management’
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