Poster (Scientific congresses and symposiums)
Termites artificially-fed on unusual diet and resulting enzymatic switches
Bauwens, Julien; Tarayre, Cédric; Matteotti, Christel et al.
201224th International Congress of Entomology
 

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Keywords :
enzymatic activity; lower termites; symbionts; starch; cellulose
Abstract :
[en] Wood-feeding termites as Reticulitermes santonensis generally feed on cellulose, hemicelluloses and lignin. However, these opportunistic insects are also able to degrade other carbohydrates, such as starch. The production of putative endogenous α - amylase has been previously shown in R. flavipes, as the disappearance of the major symbiotic flagellates from the hindgut. Here, we compared enzymatic activities (CMCase, MCCase, xylanase, amylase, α- and β-glucosidase) between different fractions of the digestive tract of starch-, cellulose-, and wood-fed termites. Main compounds of the artificial diets, namely starch or MCC, resulted in differential enzymatic activity. Even the substitution of wood by artificial diets itself seemed to induce changes in enzymatic activities, regardless of the main substrate in the diet, as we observed strong midgut α-glucosidase activity only for artificially-fed termites. Preliminary assays to isolate and characterize enzymes were performed using proteomic methods.
Disciplines :
Entomology & pest control
Author, co-author :
Bauwens, Julien ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Tarayre, Cédric ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Matteotti, Christel
Brasseur, Catherine ;  Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Destain, Jacqueline ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Vandenbol, Micheline ;  Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique
Portetelle, Daniel ;  Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique
Thonart, Philippe ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
De Pauw, Edwin  ;  Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Haubruge, Eric  ;  Université de Liège - ULiège > Services administratifs généraux > Vice-Recteur de Gembloux Agro Bio Tech
Francis, Frédéric  ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Language :
English
Title :
Termites artificially-fed on unusual diet and resulting enzymatic switches
Publication date :
August 2012
Event name :
24th International Congress of Entomology
Event date :
du 19/08/2012 au 25/08/2012
Audience :
International
Available on ORBi :
since 02 May 2013

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