Article (Scientific journals)
Persistence of an intact endometrial matrix and vessels structure in women exposed to VA-2914, a selective progesterone receptor modulator.
Ravet, Stéphanie; Munaut, Carine; Blacher, Silvia et al.
2008In Journal of Clinical Endocrinology and Metabolism, 93 (11), p. 4525-31
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Keywords :
Endometrium; image analysis; PRMs; VA-2914; vascularisation
Abstract :
[en] Background: VA-2914 is a selective progesterone receptor modulator with potential contraceptive activity that induces amenorrhea, whereas progestins cause endometrial spotting and bleeding. This abnormal bleeding due to progestins is a consequence of focal stromal proteolysis by an increase in naked vessel size and density. Objective: Our objective was to quantify the effects of VA-2914 on endometrial vascularization, fibrillar matrix, and vascular endothelial growth factor (VEGF)-A expression in endometrial biopsies from 41 women before and after 12 wk daily treatment with a placebo, or 2.5, 5, or 10 mg VA-2914. Methods: Collagen fibrillar network was stained by silver impregnation. Vessel area, density, and structure were quantified with a computer-assisted image analysis system after double immunostaining using an anti-von Willebrand factor (endothelial cells) and an anti- smooth muscle actin (vascular smooth muscle cells) marker antibody. VEGF-A mRNAs were quantified by RT-PCR and localized by immunohistochemistry. Results: The endometrial vessels, collagen network, and mRNA levels of VEGF-A were identical during the luteal phase at baseline and in VA-2914 treated women. VEGF-A distribution was unchanged. Conclusions: VA-2914 does not alter the endometrial matrix and cells, and does not modify the endometrial vessel morphology as compared with baseline biopsies.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Ravet, Stéphanie ;  Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Obstétrique CHR
Munaut, Carine  ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Blacher, Silvia ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Brichant, Géraldine ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Labied, Soraya ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Beliard, Aude ;  Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Obstétrique CHR
Chabbert-Buffet, N.
Bouchard, P.
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement
Pintiaux, Axelle ;  Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Obstétrique CHR
Language :
English
Title :
Persistence of an intact endometrial matrix and vessels structure in women exposed to VA-2914, a selective progesterone receptor modulator.
Publication date :
2008
Journal title :
Journal of Clinical Endocrinology and Metabolism
ISSN :
0021-972X
eISSN :
1945-7197
Publisher :
Endocrine Society, Chevy Chase, United States - Maryland
Volume :
93
Issue :
11
Pages :
4525-31
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 16 June 2009

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