[en] Homeodomain-containing proteins are transcription factors regulating the coordinated expression of multiple target genes involved in development, differentiation and cellular transformation. In this study, we demonstrated that HOXB7, one member of this family, behaved as a transactivator in breast cancer cells. Deletion of either the HOXB7 N-terminal domain or the C-terminal acidic tail abolished this transcriptional effect, suggesting a combination of distinct functional transactivating domains. HOXB7 physically interacted both in vitro and in vivo with the coactivator CREB-binding protein (CBP). This interaction led to an enhanced transactivating potential and required the N-terminal of HOXB7 as well as two domains located at the C-terminal part of CBP. Moreover, trichostatin A, a deacetylase inhibitor, strongly enhanced the transcriptional properties of HOXB7. Our data therefore indicate that HOX proteins can directly interact with CBP and that acetylation/deacetylation may regulate their transcriptional properties.
Research center :
Giga-Signal Transduction - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Chariot, Alain ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Van Lint, Carine
Chapelier, Muriel
Gielen, Jacques
Merville, Marie-Paule ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Bours, Vincent ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Génétique générale et humaine
Language :
English
Title :
CBP and histone deacetylase inhibition enhance the transactivation potential of the HOXB7 homeodomain-containing protein
Publication date :
1999
Journal title :
Oncogene
ISSN :
0950-9232
eISSN :
1476-5594
Publisher :
Nature Publishing Group, Basingstoke, United Kingdom
Volume :
18
Pages :
4007-4012
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] Télévie [BE]
Arany Z, Newsome D, Oldread E, Livingston DM and Eckner R. (1995). Nature, 374, 81-84.
Avantaggiati ML, Ogryzko V, Gardner K, Giordano A, Levine AS and Kelly K. (1997). Cell, 89, 1175-1184.
Bannister AJ and Kouzarides T. (1996). Nature, 384, 641-643.
Care A, Silvani A, Meccia E, Mattia G, Stoppacciaro A, Parmiani G, Peschle C and Colombo MP. (1996). Mol. Cell. Biol., 16, 4842-4851.
Chakravarti D, LaMorte VJ, Nelson MC, Nakajima T, Schulman IG, Juguilon H, Montminy M and Evans RM. (1996). Nature, 383, 99-103.
Chang CP, Shen WF, Rozenfeld S, Lawrence HJ, Largman C and Cleary M. (1995). Genes Dev., 9, 663-674.
Chang CP, De Vito I and Cleary ML. (1997). Mol. Cell. Biol., 17, 81-88.
Chariot A. Senterre-Lesenfants S, Sobel ME and Castronovo V. (1998). J. Cell. Biochem., 71, 46-54.
Chrivia JC, Kwok RP, Lamb N, Hagiwara M, Montminy MR and Goodman R. (1993). Nature. 365, 855-859.
Corsetti MT, Briata P, Sanseverino L, Daga A, Airoldi I, Simeone A, Palmisano G, Angelini C, Boncinelli E and Corte G. (1992). Nucleic Acid Res., 20, 4465-4472.
Deguchi Y, Moroney JF and Kehrl J. (1991). Blood, 78, 445-450.
Eckner R, Ewen ME, Newsome D, Gerdes M, DeCaprio JA, Lawrence JB and Livingston DM. (1994). Genes Dev., 8, 869-884.
Favier B and Dollé P. (1997). Mol. Hum. Reprod., 3, 115-131.
Feng XH, Zhang Y, Wu RY and Derynck R. (1998). Genes Dev., 12, 2153-2163.
Gehring WJ, Affolter M and Bürglin T. (1994). Ann. Rev. Biochem., 63, 487-526.
Giles RH, Peters DJ and Breuning MH. (1998). Trends Genet., 14, 178-183.
Gu W and Roeder RG. (1997a). Nature, 387, 819-823.
Gu W and Roeder RG. (1997b). Cell, 90, 595-606.
Hanstein B, Eckner R, Direnzo J, Halachmi S, Liu H, Searcy B, Kurokawa R and Brown M. (1996). Proc. Natl. Acad. Sci. USA, 93, 11540-11545.
Imhof A, Yang X-J, Ogryzko VV, Nakatani Y, Wolffe AP and Ge H. (1997). Curr. Biol., 7, 689-692.
Janknecht R, Wells NJ and Hunter T. (1998). Genes Dev., 12, 2114-2119.
Kaelin Jr WG, Pallas DC, DeCaprio JA, Kaye FJ and Livingston DM. (1991). Cell, 64, 521-532.
Kamei Y, Xu L, Heinzel T, Torchia J, Kurowaka R, Gloss B, Lin SC, Heyman RA, Rose DW, Glass CK and Rosenfeld MG. (1996). Cell, 85, 403-414.
Kawasaki H, Eckner R, Yao TP, Taira K, Chiu R, Livingston DM and Yokoyama KK. (1998). Nature, 393, 284-289.
Levine M and Hoey T. (1988). Cell, 55, 537-540.
Li H and Don Chen J. (1998). J. Biol. Chem., 273, 5948-5954.
Petríj F, Giles RH, Dauwerse HG, Saris JJ, Hennekam RCM, Masuno M, Tommerup N, van Ommen GJB, Goodman RH, Peters DJM and Breuning MH. (1995). Nature, 376, 348-351.
Sanlioglu S, Zhang X, Baader SL and Oberdick J. (1998). J. Neurobiol., 36, 559-571.
Sauer F and Tjian R. (1997). Curr. Opin. Genet. & Dev., 7, 176-181.
Schnabel CA and Abate-Shen C. (1996). Mol. Cell. Biol., 16, 2678-2688.
Shen WF, Largman C, Lowney P, Corral JC, Detmer K, Hauser CA, Simonitch TA, Hack FM and Lawrence HJ. (1989). Proc. Natl. Acad. Sci. USA, 86, 8536-8540.
Shen WF, Montgomery JC, Rozenfeld S, Moskow JJ, Jeffrey Lawrence H, Buchberg AM and Largman C. (1997). Mol. Cell. Biol., 17, 6448-6458.
Shikama N, Lyon J and La Thangue NB. (1996). Trends Cell Biol., 7, 230-236.
Shimamoto T, Ohyashiki K, Toyama K and Takeshita K. (1998). Int. J. Hematol., 67, 339-350.
Simeone A, Mavilio F, Acampora D, Giampaolo A, Paiella A, Zappavigna V, D'Esposito M, Pannese M, Russo G, Boncinelli E and Peschle C. (1987). Proc. Natl. Acad. Sci. USA, 84, 4914-4918.
Smith CL, Onate SA, Tsai MJ and O'Mailley BW. (1996). Proc. Natl. Acad. Sci. USA, 93, 8884-8888.
Sowa Y, Orita T, Minamikawa S, Nakano K, Mizuno T, Nomura H and Sakai T. (1997). Biochem. Biophys. Res. Commun., 241, 142-150.
Tanaka Y, Naruse I, Mackawa T, Masuya H, Shiroishi T and Ishii S. (1997). Proc. Natl. Acad. Sci. USA, 94, 10215-10220.
Yang XJ, Ogryzko VV, Nishikawa JI, Howard BH and Nakatani Y. (1996). Nature, 382, 319-324.
Vigano MA, Di Rocco G, Zappavigna V and Mavilio F. (1998). Mol. Cell. Biol., 18, 6201-6212.
Xu L, Lavinsky RM, Dasen JS, Flynn SE, McInerney EM, Mullen TM, Heinzel T, Szeto D, Korzus E, Kurokawa R, Aggarwal AK, Rose DW, Glass CK and Rosenfeld MG. (1998). Nature, 395, 301-306.
Zappavigna V, Sartori D and Mavilio F. (1994). Genes Dev., 8, 732-744.
Zhang N, Shen WF, D Ho A and Lu M. (1996). Oncogene, 13, 1781-1787.
Zhang W and Bieker JJ. (1998). Proc. Natl. Acad. Sci. USA, 95, 9855-9860.