Reference : Induce triploidy by heat shock in Eurasian perch, Perca fluviatilis
Scientific journals : Article
Life sciences : Aquatic sciences & oceanology
http://hdl.handle.net/2268/14524
Induce triploidy by heat shock in Eurasian perch, Perca fluviatilis
English
Rougeot, Carole mailto [Université de Liège - ULg > > Centre de formation et de recherche en aquaculture (CEFRA) >]
Minet, Laurent [> > > >]
Prignon, Christian mailto [Université de Liège - ULg > > Centre de formation et de recherche en aquaculture (CEFRA) >]
Vanderplasschen, Alain mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
Detry, Bruno [Université de Liège - ULg > > Immunologie et vaccinologie >]
Pastoret, Paul-Pierre mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires >]
Mélard, Charles mailto [Université de Liège - ULg > > Centre de formation et de recherche en aquaculture (CEFRA) >]
2003
Aquatic Living Resources
Gauthier-Villars/Editions Elsevier
16
2, MAR-APR
90-94
Yes (verified by ORBi)
International
0990-7440
Paris
[en] ploidy induction ; heat shocks ; Perca fluviatilis
[en] In Eurasian perch (Perca fluviatilis), females grow significantly faster than males. Moreover, gonadal development has a significant negative impact on somatic growth and fillet yield. In order to induce sterility, triploidy induction was attempted by subjecting fertilised eggs to heat shocks. Different combinations of temperature (28, 30, 34, 35 and 36 degreesC), duration (2, 5, 10 and 25 min) and time of shock initiation (TI = 3, 5 and 7 min post-fertilisation) were tested. Flow cytometry analysis was used to assess ploidy level of control and heat-shocked larvae. Low intensity (28-30 degreesC) and long duration (10 and 25 min) shocks lead to significantly higher survival (44 +/- 26%) and triploidisation (71 +/- 26%) rates than high intensity (34-36 degreesC) and short duration (2 and 5 min) shocks (17 +/- 19% and 21 +/- 26%, respectively). The most effective conditions for efficient triploidy induction were low intensity shock of 30 degreesC, applied 5 min post-fertilisation for 25 min. This treatment led to the production of all-triploid populations (100%) with up to 43% survival rate. (C) 2003 Editions scientifiques et medicales Elsevier SAS and Ifremer/IRD/Inra/Cemagref. All rights reserved.
http://hdl.handle.net/2268/14524

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