Reference : Gynogenesis induction and sex determination in the Eurasian perch, Perca fluviatilis
Scientific journals : Article
Life sciences : Aquatic sciences & oceanology
http://hdl.handle.net/2268/14522
Gynogenesis induction and sex determination in the Eurasian perch, Perca fluviatilis
English
Rougeot, Carole mailto [Université de Liège - ULg > > Centre de formation et de recherche en aquaculture (CEFRA) >]
Ngingo, J. V. [> > > >]
Gillet, Laurent mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
Vanderplasschen, Alain mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
Mélard, Charles mailto [Université de Liège - ULg > > Centre de formation et de recherche en aquaculture (CEFRA) >]
3-Jan-2005
Aquaculture
Elsevier Science Bv
243
1-4
411-415
Yes (verified by ORBi)
International
0044-8486
Amsterdam
[en] gynogenesis ; sex determination ; Perca fluviatilis
[en] In the present study, we used meiotic gynogenesis, widely used in studies on sex determination, to confirm female homogamety in Eurasian perch, Perca fluviatilis. Sperm irradiated with UV for 400 s was used to artificially fertilized eggs. The diploid of the resulting embryos was restored by a heat shock (30 degreesC) applied to the eggs 5 min postfertilization, for 25 min. Fertilization (ranging between 45% and 75%) and survival rates at hatching (ranging between 3.4% and 46.6%) were not significantly different (P>0.05) between the diploid control and gynogenetics. The diploid controls and two batches of gynogenetics contained 100% diploid larvae, whereas two other batches of gynogenetics contained 6.7% and 10.0% triploid larvae. The sex ratios of the diploid controls were not significantly different from 1:1, whereas all gynogenetic families were 100% female. These results confirm female homogamety in Eurasian perch, demonstrated by the use of hormonally mascilinized breeders in a previous study. (C) 2004 Elsevier B.V. All rights reserved.
http://hdl.handle.net/2268/14522

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