Article (Scientific journals)
Impact of bone marrow-derived mesenchymal stromal cells on experimental xenogeneic graft-versus-host disease
Bruck, France; Belle, Ludovic; LECHANTEUR, Chantal et al.
2013In Cytotherapy, 15 (3), p. 267-279
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Keywords :
graft-versus-host desease; GVHD; mesenchymal stromal cells; MSCs; NOD/SCID mice; NSG mice
Abstract :
[en] Background aims. Graft-versus-host disease (GVHD) is a life-threatening complication of allogeneic hematopoietic cell transplantation caused by donor T cells reacting against host tissues. Previous studies have suggested that mesenchymal stromal cells (MSCs) could exert potent immunosuppressive effects. Methods. The ability of human bone marrow derived MSCs to prevent xenogeneic GVHD in non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice and in NOD/SCID/interleukin-2Rg(null) (NSG) mice transplanted with human peripheral blood mononuclear cells (PBMCs) was assessed. Results. Injection of 200 106 human PBMCs intraperitoneally (IP) into sub-lethally (3.0 Gy) irradiated NOD/SCID mice also given anti-asialo GM1 antibodies IP 1 day prior and 8 days after transplantation induced lethal xenogeneic GVHD in all tested mice. Co-injection of 2 106 MSCs IP on day 0 did not prevent lethal xenogeneic GVHD induced by injection of human PBMCs. Similarly, injection of 30 106 human PBMCs IP into sub-lethally (2.5 Gy) irradiated NSG mice induced a lethal xenogeneic GVHD in all tested mice. Injection of 3 106 MSCs IP on days 0, 7, 14 and 21 did not prevent lethal xenogeneic GVHD induced by injection of human PBMCs. Conclusions. Injection of MSCs did not prevent xenogeneic GVHD in these two humanized mice models.
Disciplines :
Hematology
Author, co-author :
Bruck, France 
Belle, Ludovic  ;  Université de Liège > GIGA-R : Hématologie
LECHANTEUR, Chantal ;  Centre Hospitalier Universitaire de Liège - CHU > Thérapie cellulaire
de Leval, Laurence
Hannon, Muriel ;  Université de Liège - ULiège > GIGA-R : Hématologie
DUBOIS, Sophie ;  Centre Hospitalier Universitaire de Liège - CHU > Thérapie cellulaire
CASTERMANS, Emilie ;  Centre Hospitalier Universitaire de Liège - CHU > Génétique
Humblet-Baron, Stéphanie
Rahmouni, Souad  ;  Université de Liège - ULiège > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén.
Beguin, Yves  ;  Université de Liège - ULiège > GIGA-R : Hématologie
BRIQUET, Alexandra  ;  Centre Hospitalier Universitaire de Liège - CHU > Centre d'oncologie
Baron, Frédéric   ;  Université de Liège - ULiège > GIGA-R : Hématologie
 These authors have contributed equally to this work.
Language :
English
Title :
Impact of bone marrow-derived mesenchymal stromal cells on experimental xenogeneic graft-versus-host disease
Publication date :
March 2013
Journal title :
Cytotherapy
ISSN :
1465-3249
eISSN :
1477-2566
Publisher :
Taylor & Francis, London, United Kingdom
Volume :
15
Issue :
3
Pages :
267-279
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 13 March 2013

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