Article (Scientific journals)
Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection
Loret, S.; Nollevaux, G.; Remacle, R. et al.
2004In Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 805 (2), p. 195-202
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Keywords :
poly(ethylene glycol); intestinal permeability; biomaterial; biocompatibility; biioassay
Abstract :
[en] We developed a treatment of urine samples allowing the analysis of two intestinal permeability markers: polyethylene glycol (PEG) 400 (highly diffusible; basal permeability indicator) and PEG 4000 (poorly diffusible; indicator of an abnormal increase of permeability) by a unique gel permeation chromatography (GPC) with refractometric detection. Urinary PEG were extracted using a mixed-bed resin composed of C2 and C 18 layers. Permeability mean values determined in 11 human healthy subjects were 24.20 +/- 9.30% and 0.12 +/- 0.08% for, respectively, PEG 400 and 4000. The percentage of the PEG 4000 permeability value to the one of PEG 400 corresponded to an intestinal permeability index (IPI) of 0.52 +/- 0.35 expressing a low diffusion of this poorly permeability marker. (C) 2004 Published by Elsevier B.V.
Research center :
CEIB - Centre Interfacultaire des Biomatériaux - ULiège
Disciplines :
Chemistry
Biochemistry, biophysics & molecular biology
Author, co-author :
Loret, S.
Nollevaux, G.
Remacle, R.
Klimek, Monique ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Barakat, I.
Deloyer, P.
Grandfils, Christian ;  Université de Liège - ULiège > Biochimie et physiologie générale
Dandrifosse, Guy ;  Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Language :
English
Title :
Analysis of PEG 400 and 4000 in urine for gut permeability assessment using solid phase extraction and gel permeation chromatography with refractometric detection
Publication date :
15 June 2004
Journal title :
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
ISSN :
1570-0232
eISSN :
1873-376X
Publisher :
Elsevier Science Bv, Amsterdam, Netherlands
Volume :
805
Issue :
2
Pages :
195-202
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Contrôle de la perméabilité intestinale aux macromolécules”, développement d’un système diagnostique. Travaux menés en collaboration avec le Professeur L. Delattre et le Professeur J. Belaiche. Travaux de recherche subsidiés par la Région Wallonne. (convention ULg –RW : n° 9813722) Année 1999-2003
Funders :
Région wallonne [BE]
Available on ORBi :
since 12 March 2013

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