atomic force microscopy; DNA; gene therapy vehicle; Dynamic Light Scattering
Abstract :
[en] Atomic force microscopy (AFM) is used to describe the formation process of polymer/DNA complexes. Two main objectives of this research are presented. The first one is to apply AFM as an effective tool to analyse DNA molecules and different polycation/DNA complexes in order to evaluate their degree of condensation (size and shape). The other one is to search for a relationship between the condensation state of DNA and its transfection efficiency. In this study, linear methacrylate based polymers and globular SuperFect polymers are used in order to induce DNA condensation. Ternary complexes, composed of methacrylate based polymers and polyethylene glycol (PEG)-based copolymers, are also investigated. AFM allows us to confirm good condensation conditions and relate them (or not) to transfection efficiencies. These AFM results (obtained after drying in air) are compared with measurements deduced from Dynamic Light Scattering (DLS) experiments performed in water. This comparison allowed us to identify the structural modifications resulting from deposition on the mica surface. (c) 2006 Elsevier B.V. All rights reserved.
Research center :
CEIB - Centre Interfacultaire des Biomatériaux - ULiège
Disciplines :
Microbiology Biotechnology
Author, co-author :
Volcke, C.
Pirotton, S.
Grandfils, Christian ; Université de Liège - ULiège > Biochimie et physiologie générale
Humbert, C.
Thiry, P. A.
Ydens, I.
Dubois, Philippe ; Université de Liège - ULiège > Centre d'études et de rech. sur les macromolécules (CERM)
Raes, M.
Language :
English
Title :
Influence of DNA condensation state on transfection efficiency in DNA/polymer complexes: An AFM and DLS comparative study
Publication date :
20 August 2006
Journal title :
Journal of Biotechnology
ISSN :
0168-1656
eISSN :
1873-4863
Publisher :
Elsevier Science Bv, Amsterdam, Netherlands
Volume :
125
Issue :
1
Pages :
11-21
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Nouveaux polycations et vectorisation d’ADN dans les cellules de parois vasculaires” Convention Région Wallonne n° 14612, année 2001-2004 dans le cadre du concours Initiative 2000
Allen M.J., Bradbury E.M., and Balhorn R. AFM analysis of DNA-protamine complexes bound to mica. Nucleic Acids Res. 25 (1997) 2221-2226
Anselmetti D., Dreier M., Luthi R., Richmond T., Meyer E., Frommer J., and Gunterodt H.-J. Biological materials studied with dynamic force microscopy. J. Vac. Sci. Technol. B 12 (1994) 1500-1503
Argaman M., Golan R., Thomson N.H., and Hansma H.G. Phase imaging of moving DNA molecules and DNA molecules replicated in the atomic force microscope. Nucleic Acids Res. 25 (1997) 4379-4384
Cherng J.-Y., van de Wetering P., Talsma H., Crommelin D.A., and Hennink W.E. Effect of size and serum proteins on transfection efficiency of poly((2-dimethylamino)ethyl methacrylate)-plasmid nanoparticles. Pharm. Res. 13 (1996) 1038-1042
Choi J.S., Lee E.J., Choi Y.H., Jeong Y.J., and Park J.S. Poly(ethylene glycol)-block-poly(l-lysine) dendrimer: novel linear polymer/dendrimer block copolymer forming a spherical water-soluble polyionic complex with DNA. Bioconjug. Chem. 10 (1999) 62-65
Congiu A., Pozzi D., Esposito C., Castellano C., and Mossa G. Correlation between structure and transfection efficiency: a study of DC-Chol-DOPE/DNA complexes. Colloids Surf. B Biointerfaces 36 (2004) 43-48
Dunlap D.D., Maggi A., Soria M.R., and Monaco L. Nanoscopic structure of DNA condensed for gene delivery. Nucleic Acids Res. 25 (1997) 3095-3101
Feldman S.H. Components of gene therapy experimentation that contribute to relative risk. Comp. Med. 53 (2003) 147-158
Frechet J.M. Functional polymers and dendrimers: reactivity, molecular architecture and interfacial energy. Science 263 (1994) 1710-1715
Gao X., and Huang L. Potentiation of cationic liposome-mediated gene delivery by polycations. Biochemistry 35 (1996) 1027-1036
Hansma H.G., Vesenka J., Siegerist C., Kelderman G., Morrett H., Sinsheimer R.L., Elings V., Bustmante C., and Hansma P.K. Reproducible imaging and dissection of plasmid DNA under liquid with the atomic force microscope. Science 256 (1992) 1180-1184
Hansma H.G., Revenko I., Kim K., and Laney D.E. Atomic force microscopy of long and short double-stranded, single-stranded and triple-stranded nucleic acids. Nucleic Acids Res. 24 (1996) 713-720
Hansma H.G., Golan R., Hsieh W., Lollo C.P., Mullen-Ley P., and Kwoh D. DNA condensation for gene therapy as monitored by atomic force microscopy. Nucleic Acids Res. 26 (1998) 2481-2487
Howard K.A., Dash P.R., Read M.L., Ward K., Tomkins L.M., Nazarova O., Ulbrich K., and Seymour L.W. Influence of hydrophilicity of cationic polymers on the biophysical properties of polyelectrolyte complexes formed by self-assembly with DNA. Biochim. Biophys. Acta 1475 (2000) 245-255
Itaka K., Yamauchi K., Harada A., Nakamura K., Kawaguchi H., and Kataoka K. Polyion complex micelles from plasmid DNA and poly(ethylene glycol)-poly(l-lysine) block copolymer as serum-tolerable polyplex system: physicochemical properties of micelles relevant to gene transfection efficiency. Biomaterials 24 (2003) 4495-4506
Jones N.A., Hill I.R.C., Stolnik S., Bignotti F., Davis S.S., and Garnett M.C. Polymer chemical structure is a key determinant of physicochemical and colloidal properties of polymer-DNA complexes for gene delivery Biochim. Biophys. Acta 1517 (2000) 1-18
Lyubchenko Y., Shlyakhtenko L., Harrington R., Oden P., and Lindsay S. Atomic force microscopy of long DNA: imaging in air and under water. Proc. Natl. Acad. Sci. U.S.A. 90 (1993) 2137-2140
Lim Y., Kim S., Suh H., and Park J. Biodegradable, endosome disruptive, and cationic network-type polymer as a highly efficient and nontoxic gene delivery carrier. Bioconjug. Chem. 13 (2002) 952-957
Lin Z., Wang C., Feng X., Liu M., Li J., and Bai C. The observation of the local ordering characteristics of spermidine-condensed DNA: atomic force microscopy and polarizing microscopy studies. Nucleic Acids Res. 26 (1998) 3228-3234
Maeda Y., Matsumoto T., and Kawai T. Observation of single- and double-stranded DNA using non-contact atomic force microscopy. Appl. Surf. Sci. 140 (1999) 400-405
Maguire-Zeiss K.A., and Federoff H.J. Safety of viral vectors for neurological gene therapies. Curr. Opin. Mol. Ther. 6 (2004) 473-481
Maksimenko A.V., Mandrouguine V., Gottikh M.B., Bertrand J.-R., Majoral J.-P., and Malvy C. Optimisation of dendrimer-mediated gene transfer by anionic oligomers. J. Gene Med. 5 (2003) 61-71
Martin A.L., Davies M.C., Rackstraw B.J., Roberts C.J., Stolnik S., Tendler S.J.B., and Williams P.M. Observation of DNA-polymer condensate formation in real time at a molecular level. FEBS Lett. 480 (2000) 106-112
Miller A.D. The problem with cationic liposome/micelle- based non-viral vector systems for gene therapy. Curr. Med. Chem. 10 (2003) 1195-1211
Moreno-Herrero F., Colchero J., and Baro A.M. DNA height in scanning force microscopy. Ultramicroscopy 96 (2003) 167-174
Van Noort S.J.T., Van der Werf K.O., De Grooth B.G., Van Hulst N.F., and Greve J. Height anomalies in tapping mode atomic force microscopy in air caused by adhesion. Ultramicroscopy 69 (1997) 117-127
Pantoustier N., Moins S., Wautier M., Degée P., and Dubois P. Solvent-free synthesis and purification of poly[2-(dimethylamino)ethyl methacrylate] by atom transfer radical polymerisation. Chem. Commun. 3 (2003) 340-342
Pirotton S., Muller C., Pantoustier N., Botteman F., Collinet S., Grandfils C., Dandrifosse G., Degée P., Dubois P., and Raes M. Enhancement of transfection efficiency through rapid and noncovalent post-PEGylation of Poly(dimethylaminoethylmethacrylate)/DNA complexes. Pharm. Res. 21 (2004) 1471-1479
Rackstraw B.J., Martin A.L., Stolnik S., Roberts C.J., Garnett M.C., Davies M.C., and Tendler S.J.B. Microscopic investigations into PEG-cationic polymer-induced DNA condensation. Langmuir 17 (2001) 3185-3193
Radmacher M., Tilmann R.W., Fritz M., and Gaub H.E. From molecules to cells: imaging soft samples with the atomic force microscope. Science 257 (1992) 1900-1905
Razatos A., Ong Y.-L., Sharma M.M., and Georgiou G. Molecular determinants of bacterial adhesion monitored by atomic force microscopy. Proc. Natl. Acad. Sci. U.S.A. 95 (1998) 11059-11064
Reich Z., Kapon R., Pilpel Y., Zmora S., and Scolnik Y. Scanning force microscopy in the applied biological sciences. Biotechnol. Adv. 19 (2001) 451-485
Reschel T., Konak C., Oupicky D., Seymour L.W., and Ulbrich K. Physical properties and in vitro transfection efficiency of gene delivery vectors based on complexes of DNA with synthetic polycations. J. Control. Release 81 (2002) 201-217
Toncheva V., Wolfert M.A., Dash P.R., Oupicky D., Ulrich K., Seymour L., and Schacht E.H. Novel vectors for gene delivery formed by self-assembly of DNA with poly(l-lysine) grafted with hydrophilic polymers. Biochim. Biophys. Acta 1380 (1998) 354-368
Vijayanathan V., Thomas T., Antony T., Shirahata A., and Thomas T.J. Formation of DNA nanoparticles in the presence of novel polyamine analogues: a laser light scattering and atomic force microscopic study. Nucleic Acids Res. 32 (2004) 127-134
van de Wetering P., Cherng J.-Y., Talsma H., and Hennink W.E. Relation between transfection efficiency and cytotoxicity of poly(2-(dimethylamino)ethyl methacrylate)/plasmid complexes. J. Control. Release 49 (1997) 59-69
van de Wetering P., Cherng J.-Y., Talsma H., Crommelin D.J.A., and Hennink W.E. 2-(dimethylamino)ethyl methacrylate based (co)polymers as gene transfer agents. J. Control. Release 53 (1998) 145-153
Wilson J.M. Adenovirus-mediated gene transfer to liver. Adv. Drug Deliv. Rev. 17 (1995) 303-307
Wolfert M.A., Dash P.R., Nazarova O., Oupicky D., Seymour L.W., Smart S., Strohalm J., and Ulrich K. Polyelectrolyte vectors for gene delivery: influence of cationic polymer on biophysical properties of complexes formed with DNA. Bioconjug. Chem. 10 (1999) 993-1004
Wyman C., Grotkopp E., Bustamante C., and Nelson H.C.M. Determination of heat-shock transcription factor 2 stoichiometry at looped DNA complexes using scanning force microscopy. EMBO J. 14 (1995) 117-123
Yang J., Takeyasu K., and Shao Z. Atomic force microscopy of DNA molecules. FEBS 301 (1992) 173-176
Yang G., Vesenka J.P., and Bustamante C.J. Effects of tip-sample forces and humidity on the imaging of DNA with a scanning force microscope. Scanning 18 (1996) 344-350
