Article (Scientific journals)
Optimisation de la production de Pseudomonas fluorescens BTP1 en fermenteur.
Mputu Kanyinda, Jean-Noël; Thonart, Philippe
2013In International Journal of Microbiology Research, 5 (1), p. 370-373
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Keywords :
Bioreactor; Freeze-drying; Optimisation; Protective compound; Water activity
Abstract :
[en] The optimisation of production and freeze-drying of P. fluorescens used as a bio-control agent was investigated. P. fluorescens BTP1 was produced in a bioreactor with different against-pressure value (0.1 and 0.3 bar for bioreactor 1 and 2 respectively) and cells were harvested during the stationary phase (2 h and 4 h for bioreactor 1 and 2 respectively). A mixture of protective compounds were tested for freeze-drying, and the highest result was found for glycerol and maltodextrine (26%) followed by glycerol, maltodextrine and ascorbic acid (18.9%) and glycerol with ascorbic acid (8.5%). We observed that the survival rate is better at 4°C than at room temperature and those powders with protective compounds have a survival rate greater than the powder without protective compounds during storage.
Disciplines :
Biotechnology
Author, co-author :
Mputu Kanyinda, Jean-Noël ;  Université de Liège - ULiège > Doct. sc. agro. & ingé. biol.
Thonart, Philippe ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Language :
English
Title :
Optimisation de la production de Pseudomonas fluorescens BTP1 en fermenteur.
Publication date :
04 March 2013
Journal title :
International Journal of Microbiology Research
ISSN :
0975-5276
eISSN :
0975-9174
Publisher :
Bioinfo, Mumbaï, India
Volume :
5
Issue :
1
Pages :
370-373
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Conseil Alimentaire (CONSALIM)
Funders :
WBI - Wallonie-Bruxelles International [BE]
Available on ORBi :
since 11 February 2013

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