Article (Scientific journals)
Increased apoptotic chondrocytes in articular cartilage from adult heterozygous SirT1 mice.
Gabay, Odile; Oppenhiemer, Hanna; Meir, Hadar et al.
2012In Annals of the Rheumatic Diseases, 71 (4), p. 613-6
Peer Reviewed verified by ORBi
 

Files


Full Text
613.full.pdf
Publisher postprint (1.18 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Aggrecans/metabolism; Aging/metabolism/pathology; Animals; Apoptosis/physiology; Arthritis, Experimental/metabolism/pathology; Cartilage, Articular/metabolism/pathology; Cell Survival/physiology; Chondrocytes/metabolism/pathology; Collagen Type II/metabolism; Mice; Mice, Knockout; Osteoarthritis/metabolism/pathology; Sirtuin 1/deficiency/physiology
Abstract :
[en] OBJECTIVE: A growing body of evidence indicates that the protein deacetylase, SirT1, affects chondrocyte biology and survival. This report aims to evaluate in vivo attributes of SirT1 in cartilage biology of 129/J murine strains. METHODS: Heterozygous haploinsufficient (SirT1(+/-)) and wild-type (WT; SirT1(+/+)) 129/J mice aged 1 or 9 months were systematically compared for musculoskeletal features, scored for osteoarthritis (OA) severity, and monitored for chondrocyte apoptosis in articular cartilage. Sections of femorotibial joints were stained for type II collagen and aggrecan. Protein extracts from articular chondrocytes were isolated and immunoblotted for SirT1 and active caspase 3. RESULTS: Phenotypic observations show that, at 1 month of age, SirT1(+/-) mice were smaller than WT and showed a significant decrease in full-length SirT1 (FLSirT1; 110 kDa) protein levels. Levels of FLSirT1 were further decreased in both strains at 9 months. Immunoblot assays for 9-month-old strains revealed the presence of the inactive cleaved SirT1 variant (75 SirT1; 75 kDa) in WT mice, which was undetected in age-matched SirT1(+/-) mice. Nine-month-old SirT1(+/-) mice also showed increased OA and increased levels of apoptosis compared with age-matched WT mice. CONCLUSION: The data suggest that the presence of 75 SirT1 may prolong viability of articular chondrocytes in adult (9-month-old) mice.
Disciplines :
Rheumatology
Author, co-author :
Gabay, Odile
Oppenhiemer, Hanna
Meir, Hadar
Zaal, Kristien
Sanchez, Christelle  ;  Université de Liège - ULiège > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.)
Dvir-Ginzberg, Mona
Language :
English
Title :
Increased apoptotic chondrocytes in articular cartilage from adult heterozygous SirT1 mice.
Publication date :
2012
Journal title :
Annals of the Rheumatic Diseases
ISSN :
0003-4967
eISSN :
1468-2060
Publisher :
BMJ Publishing Group, United Kingdom
Volume :
71
Issue :
4
Pages :
613-6
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 February 2013

Statistics


Number of views
48 (0 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
90
Scopus citations®
without self-citations
77
OpenCitations
 
89

Bibliography


Similar publications



Contact ORBi