Reference : Capsular Gene Typing of Streptococcus agalactiae Compared to Serotyping by Latex Aggl...
Scientific journals : Article
Human health sciences : Laboratory medicine & medical technology
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/142113
Capsular Gene Typing of Streptococcus agalactiae Compared to Serotyping by Latex Agglutination
English
Yao, Kaihu [Aarhus University, Aarhus, Denmark > Department of Biomedicine, Faculty of Health Sciences > > >]
Poulsen, Knud [Aarhus University, Aarhus, Denmark > Department of Biomedicine, Faculty of Health Sciences > > >]
Maione, Domenico [Novartis Vaccines and Diagnostics, Siena, Italy > R&D > > >]
Rinaudo, Daniela [Novartis Vaccines and Diagnostics, Siena, Italy > R&D > > >]
Baldassarri, Lucilla [Instituto Superiore di Sanita, Rome, Italy > Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate > > >]
Telford, John [Novartis Vaccines and Diagnostics, Siena, Italy > > > >]
Skov Sørensen, Uffe B. [Aarhus University, Aarhus, Denmark > Department of Biomedicine, Faculty of Health Sciences > > >]
Members of the DEVANI Study Group []
Kilian, Mogens [Aarhus University, Aarhus, Denmarka > Department of Biomedicine, Faculty of Health Sciences > > >]
Melin, Pierrette mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Bactériologie, mycologie, parasitologie, virologie >]
28-Nov-2012
Journal of Clinical Microbiology
American Society for Microbiology (ASM)
51
2
503-507
Yes (verified by ORBi)
International
0095-1137
1098-660X
Washington
DC
[en] Streptococcus agalactiae ; Capsular gene typing ; serotyping
[en] We evaluated three different PCR-based capsular gene typing methods applied to 312 human and bovine Streptococcus agalac- tiae (group B Streptococcus [GBS]) isolates and compared the results to serotyping results obtained by latex agglutination. Among 281 human isolates 27% could not be typed by latex agglutination. All 312 isolates except 5 could be typed by the three PCR methods combined. Two of these methods were multiplex assays. Among the isolates that were typeable by both latex ag- glutination and capsular gene typing, 94% showed agreement between the two methods. However, each of the PCR methods showed limitations. One of the methods did not include all 10 recognized serotypes, one misidentified eight isolates of serotypes Ib and IV as serotype Ia, and one did not distinguish between serotypes VII and IX. For five isolates that showed aberrant pat- terns in the capsular gene typing, long-range PCR targeting the cps operon disclosed large insertions or deletions affecting the cps gene cluster. A sensitive flow cytometric assay based on serotype-specific antibodies applied to 76 selected isolates that were nontypeable by latex agglutination revealed that approximately one-half of these did express capsular polysaccharide. A proce- dure for convenient and reliable capsular gene typing to be included in epidemiological and surveillance studies of S. agalactiae is proposed.
Department of Biomedicine, Faculty of Health Sciences, Aarhus University, Aarhus, Denmark
Researchers ; Professionals
http://hdl.handle.net/2268/142113
10.1128/JCM.02417-12
FP7 ; 200481 - DEVANI - Design of a vaccine to immunize neonates against GBS infections through a durable maternal immune response

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
J. Clin. Microbiol.-2013-Yao-503-7.pdfPublisher postprint521.66 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.