Reference : Light induced functionalization of PCL-PEG block copolymers for the covalent immobili...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Chemistry
Engineering, computing & technology : Materials science & engineering
http://hdl.handle.net/2268/14158
Light induced functionalization of PCL-PEG block copolymers for the covalent immobilization of biomolecules
English
Pourcelle, Vincent [Université catholique de Louvain (UCL) > > Unité de Chimie Organique et Médicinale (CHOM) > >]
Freichels, Hélène [University of Liège (ULg) > Department of Chemistry > Center for Education and Research on Macromolecules (CERM) > >]
Stoffelbach, François [University of Liège (ULg) > Department of Chemistry > Center for Education and Research on Macromolecules (CERM) > >]
Auzély-Velty, Rachel [Grenoble, France > > Recherches sur les Macromolécules Végétales (CERMAV-CNRS) > >]
Jérôme, Christine mailto [University of Liège (ULg) > Department of Chemistry > Center for Education and Research on Macromolecules (CERM) > >]
Marchand-Brynaert, Jacqueline [Université catholique de Louvain (UCL) > > Unité de Chimie Organique et Médicinale (CHOM) > >]
13-Apr-2009
Biomacromolecules
American Chemical Society
10
4
966-974
Yes (verified by ORBi)
International
1525-7797
1526-4602
Washington
DC
[en] ring-opening polymerization (ROP) ; lactone ; époxide
[en] Functionalized poly-ε-caprolactone-block-polyethyleneglycol (PCL-PEG) amphiphilic copolymers were prepared to be constituents of nanocarriers used for the targeting of specific cells. Hence, we conceived a smooth and simple photografting methodology on these copolymers using a bifunctional molecular clip (O-succinimidyl-4-(p-azido-phenyl)butanoate). We prepared PCL-PEGs with pendent N-hydroxysuccinimide esters and studied the grafting with 3H-lysine, which radioactivity was counted by LSC. Several parameters were investigated, such as behavior of homopolymers, initial concentrations, irradiation, and incubation durations. Evidences of a “PEG directed photografting” are discussed and this selectivity could be improved by a selective solvent technique. The photografting on different PCL-PEGs revealed a dependency of the rates to the crystallinity of the copolymers. Several controls by SEC, DLS, and TEM of the treated copolymers were realized. Lastly, the coupling of α-d-mannopyranoside ligand was performed, reaching amounts of 5400 nmol/g of PCL-PEG. This derivatized PCL-PEG enters in the preparation of nanocarriers used for the targeting of antigen presenting cells.
Center for Education and Research on Macromolecules (CERM)
The "Région Wallonne" in the frame of the program "VACCINOR" ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Politique Scientifique Fédérale (Belgique) = Belgian Federal Science Policy ; The "CGRI-FNRS-CNRS" cooperation program
Researchers
http://hdl.handle.net/2268/14158
10.1021/bm900027r
http://pubs.acs.org/doi/pdf/10.1021/bm900027r

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