Reference : Polyester nanoparticles presenting mannose residues : toward the development of new v...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Chemistry
Engineering, computing & technology : Materials science & engineering
http://hdl.handle.net/2268/14157
Polyester nanoparticles presenting mannose residues : toward the development of new vaccine delivery systems combining biodegradability and targeting properties
English
Rieger, Jutta [University of Liège (ULg) et Grenoble (France) > Department of Chemistry (Liège) > Center for Education and Research on Macromolecules (CERM, Liège) et CERMAV (Grenoble) > >]
Freichels, Hélène [University of Liège (ULg) > Department of Chemistry > Center for Education and Research on Macromolecules (CERM) > >]
Imberty, Anne [Grenoble, France > > Centre de Recherches sur les Macromolécules Végétales (CERMAV-CNRS) > >]
Putaux, Jean-Luc [Grenoble, France > > Centre de Recherches sur les Macromolécules Végétales (CERMAV-CNRS) > >]
Delair, Thierry [Université Claude Bernard Lyon 1, Villeurbanne, France > > Laboratoire des Mateériaux Polymères et Biomatériaux > >]
Jérôme, Christine mailto [University of Liège (ULg) > Department of Chemistry > Center for Education and Research on Macromolecules (CERM) > >]
Auzely-Velty, Rachel [Grenoble, France > > Centre de Recherches sur les Macromolécules Végétales (CERMAV-CNRS) > >]
9-Mar-2009
Biomacromolecules
American Chemical Society
10
3
651-657
Yes (verified by ORBi)
International
1525-7797
1526-4602
Washington
DC
[en] biomaterial ; nanomedicine ; organic nanoparticle
[en] We report the synthesis of fully biodegradable polymeric nanoparticles presenting mannose residues at their surface and their interaction with lectins. A simple and versatile method was used to reach the surface functionalization of poly(d,l-lactic acid) (PLA) nanoparticles by mannose moieties: It consists in using an amphiphilic mannosylated poly(ethylene oxide)-b-poly(-caprolactone) (PEO-b-PCL) diblock copolymer as a bioresorbable surface modifier in a simple nanoprecipitation-evaporation procedure. The size and zeta potential of the nanoparticles were found to depend on the molar copolymer/PLA ratio, demonstrating the influence of the copolymer on the formation of the nanoparticles. The bioavailability of the mannose residues as specific recognition sites on the nanoparticle surface could be demonstrated by a modified enzyme-linked lectin assay (ELLA) using biotin-labeled lectins which interact specifically with α-d-mannopyrannoside derivatives. Besides specific interaction by lectin−mannose complex formation, nonspecific adsorption of the proteins on the nanoparticle surface was observed. These results were fully supported by isothermal titration calorimetry experiments which suggested that the balance between specific and nonspecific interactions can be controlled by the amount of glycosylated polymer used for the preparation of the nanoparticles. Such nanoparticles are expected to be specifically recognized by mannose receptors, which are highly expressed in cells of the immune system. The targeting properties of these carrier systems combined with their potential adjuvant effects due to their size in the range of 200−300 nm make them attractive candidates as vaccine delivery systems.
Center for Education and Research on Macromolecules (CERM)
Politique Scientifique Fédérale (Belgique) = Belgian Federal Science Policy ; The "Région Wallonne" in the frame of the project "VACCINOR" ; The European Community's Sixth Framework program in the frame of a Marie Curie fellowship ; The "CNRS" ; The "CGRI-FNRS-CNRS" in the framework of a bilateral cooperation
Researchers
http://hdl.handle.net/2268/14157
10.1021/bm801492c
http://pubs.acs.org/doi/pdf/10.1021/bm801492c

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