[en] Phagocytic cells, especially neutrophils (PMNs) are specialized in the production of reactive oxygen species
(ROS) to kill pathogenic agents, but an excessive ROS production is associated with tissue damages
and inflammatory diseases. Phagocytes are thus prime therapeutic targets to control inflammatory events
associated to ROS production. Nowadays, there is a growing interest for the use of polyphenols to modulate
the inflammatory response. The aim of this work was to study the antioxidant effect of NDS27, a
highly water-soluble form of the polyphenolic molecule curcumin, on in vitro stimulated equine PMNs
and human promyelocytic leukemia cells (HL-60). NDS27 was either pre-incubated with cells and eliminated
before their activation (intracellular effect) or let in the medium (extracellular effect). Our results
indicate that NDS27 significantly and dose-dependently (10 6 M–10 4 M) inhibited the ROS production
in both cell types without affecting their viability. NDS27 was able to cross and interact with cell membrane,
especially for HL-60 cells, while we observed a better intracellular antioxidant effect with PMNs.
The activity of myeloperoxidase (MPO) released by PMNs and HL-60 cells, was decreased by NDS27, but
more efficiently for PMNs. These results suggested that the greater efficiency of NDS27 in PMNs is due to
an inhibitory effect on cells which are more mature for ROS production, probably by targeting the
enzymes implied in respiratory burst like MPO. The modulatory effect of NDS27 on the oxidant activity
of cells involved in immune and inflammatory responses opens perspectives for a therapeutic control of
pathologies with excessive inflammatory reactions.
Research center :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Derochette, Sandrine ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Franck, Thierry ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Mouithys-Mickalad, Ange ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Deby-Dupont, Ginette
Neven, Philippe
Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
Intra- and extracellular antioxidant capacities of the new water-soluble form of curcumin (NDS27) on stimulated neutrophils and HL-60 cells
Alternative titles :
[en] Capacités antioxydantes intra et extracellulaires d'un nouveau dérivé soluble de la curcumine (NDS27) sur les neutrophiles et HL-60 stimulés.
Publication date :
January 2013
Journal title :
Chemico-Biological Interactions
ISSN :
0009-2797
eISSN :
1872-7786
Publisher :
Elsevier, Limerick, Ireland
Volume :
201
Issue :
1-3
Pages :
49-57
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
PURIFICATION DE LA NADPH OXYDASE DES NEUTROPHILES ÉQUINS : MISE AU POINT DE NOUVEAUX OUTILS POUR LA QUANTIFICATION ET LA MESURE SPÉCIFIQUE DE SON ACTIVITÉ ET POUR LA RECHERCHE D'INHIBITEURS DE L'ENZYME
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
T. Atsumi, S. Fujisawa, and K. Tonosaki Relationship between intracellular ROS production and membrane mobility in curcumin- and tetrahydrocurcumin-treated human gingival fibroblasts and human submandibular gland carcinoma cells Oral Dis. 11 2005 236 242
G. Bjelakovic, D. Nikolova, L.L. Gluud, R.G. Simonetti, and C. Gluud Mortality in randomized trials of antioxidant supplements for primary and secondary prevention JAMA: J. Am. Med. Assoc. 297 2007 842 857
E.J. Bland, T. Keshavarz, and C. Bucke The influence of small oligosaccharides on the immune system Carbohyd. Res. 339 2004 1673 1678
T.R. Breitman, S.E. Selonick, and S.J. Collins Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid Proc. Natl. Acad. Sci. 77 1980 2936 2940
F. Caldefie-Chézet, S. Walrand, C. Moinard, A. Tridon, J. Chassagne, and M.-P. Vasson Is the neutrophil reactive oxygen species production mesured by luminol and lucigenin chimiluminescence intra or extracellular? Comparison with DCFH-DA flow cytometry and cytochrome c reduction Clin. Chim. Acta 319 2002 9 17
D. Castagne, B. Evrard, B. Nusgens, and R. Piel Effect of beta-cyclodextrin and its derivatives on caveolae disruption, relationships with their cholesterol extraction capacities J. Incl. Phenom. Macro. Chem. 67 2010 8
S. Collins The HL-60 promyelocytic leukemia cell line: proliferation, differentiation, and cellular oncogene expression Blood 70 1987 1233 1244
C. Dahlgren, and A. Karlsson Respiratory burst in human neutrophils J. Immunol. Meth. 232 1999 3 14
G. Deby-Dupont, A. Mouithys-Mickalad, D. Serteyn, M. Lamy, and C. Deby Resveratrol and curcumin reduce the respiratory burst of Chlamydia-primed THP-1 cells Biochem. Biophys. Res. Commun. 333 2005 21 27
W. Dröge Free radicals in the physiological control of cell function Physiol. Rev. 82 2002 47 95
T. Franck, S. Grulke, G. Deby-Dupont, C. Deby, H. Duvivier, F. Peters, and D. Serteyn Development of an enzyme-linked immunosorbent assay for specific equine neutrophil myeloperoxidase measurement in blood J. Vet. Diagn. Invest. 17 2005 412 419
T. Franck, S. Kohnen, G. de la Rebière, G. Deby-Dupont, C. Deby, A. Niesten, and D. Serteyn Activation of equine neutrophils by phorbol myristate acetate or N-formyl-methionyl-leucyl-phenylalanine induces a different response in reactive oxygen species production and release of active myeloperoxidase Vet. Immunol. Immunop. 130 2009 243 250
T. Franck, S. Kohnen, G. Deby-Dupont, S. Grulke, C. Deby, and D. Serteyn A specific method for measurement of equine active myeloperoxidase in biological samples and in in vitro tests J. Vet. Diagn. Invest. 18 2006 326 334
T. Franck, S. Kohnen, S. Grulke, P. Neven, Y. Goutman, F. Péters, B. Pirotte, G. Deby-Dupont, and D. Serteyn Inhibitory effect of curcuminoids and tetrahydrocurcuminoids on equine activated neutrophils and myeloperoxidase activity Physiol. Res. 57 2008 577 587
T. Franck, S. Kohnen, K. Zouaoui, P.V. Antwerpen, A. Bosseloir, A. Niesten, O. Gash, M. Nys, G. Deby-Dupont, and D. Serteyn A new easy method for specific measurement of active myeloperoxidase in human biological fluids and tissue extract Talanta 80 2009 723 729
K.E. Heim, A.R. Tagliaferro, and D.J. Bobilya Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships The J. Nut. Biochem. 13 2002 572 584
W.-C. Hung, F.-Y. Chen, C.-C. Lee, Y. Sun, M.-T. Lee, and H.W. Huang Membrane-thinning effect of curcumin Biophys. J. 94 2008 4331 4338
H.I. Ingolfsson, R.E. Koeppe, and O.S. Andersen Curcumin is a modulator of bilayer material properties Biochemistry 46 2007 10384 10391
J.S. Jurenka Anti-inflammatory properties of curcumin, a major constituent of Curcuma longa: a review of preclinical and clinical research Altern. Med. Rev. 14 2009 141 153
H. Kikuchi, F. Kuribayashi, N. Kiwaki, and T. Nakayama Curcumin dramatically enhance retinoic acid-induced superoxide generating activity via accumulation of p47-phox and p67-phox proteins in U937 cells Biochem. Biophys. Res. Commun. 395 2010 61 65
S. Klebanoff Myeloperoxidase: friend and foe J. Leucocyte Biol. 77 2005 598 625
R. Kohen, and A. Nyska Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification Toxicol. Pathol. 30 2002 620 650
V. Kumar, and A. Sharma Neutrophils: Cinderella of innate immune system Int. Immunopharmacol. 10 2010 1325 1334
C. Lin, and H. Wang NADPH oxidase is involved in H2O 2-induced differentiation of human promyelocytic leukaemia HL-60 cells Cell Biol. Int. 36 2012 391 395
J.-Y. Liu, S.-J. Lin, and J.-K. Lin Inhibitory effects of curcumin on protein kinase C activity induced by 12-O-tetradecanoyl-phorbol-13-acetate in NIH 3T3 cells Carcinogenesis 14 1993 857 861
R.K. Maheshwari, A.K. Singh, J. Gaddipati, and R.C. Srimal Multiple biological activities of curcumin: a short review Life Sci. 78 2006 2081 2087
T. Masuda, T. Maekawa, K. Hidaka, H. Bando, Y. Takeda, and H. Yamaguchi Chemical studies on antioxidant mechanism of curcumin: analysis of oxidative coupling products from curcumin and linoleate J. Agri. Food Chem. 49 2001 2539 2547
E.R. Miller, R. Pastor-Barriuso, D. Dalal, R.A. Riemersma, L.J. Appel, and E. Guallar Meta-analysis: high-dosage vitamin E supplementation may increase all-cause mortality Annal. Internal Med. 142 2005 37 46
G. Minguet, G. de la Rebiere, T. Franck, J. Joris, D. Serteyn, and C. Sandersen Sevoflurane inhibits equine myeloperoxidase release and activity in vitro Vet. Anaesth. Analg. 11 2012 1467 2995
J. Miquel, A. Bernd, J.M. Sempere, J. Diaz-Alperi, and A. Ramirez The curcuma antioxidants: pharmacological effects and prospects for future clinical use Rev., Arch. Gerontol. Geriatr. 34 2002 37 46
A. Murakami, and H. Ohigashi Targeting NOX, INOS and COX-2 in inflammatory cells: chemoprevention using food phytochemicals Int. J. Cancer 121 2007 2357 2363
O. Myhre, J.M. Andersen, and H.A.F. Fonnum Evaluation of the probes 2′,7′-dichlorofluorescin diacetate, luminol, and lucigenin as indicators of reactive species formation Biochem. Pharmacol. 65 2003 1575 1582
Y. Nakamura, A. Murakami, and H. Ohigashi Search for naturally-occurring antioxidative chemopreventors on the basis of the involvement of leukocyte-derived reactive oxygen species in carcinogenesis Asian Pacific J. Cancer Prev. 1 2000 115 120
P. Neven, D. Serteyn, J. Delarge, R. Kiss, V. Mathieu, D. Cataldo, N. Rocks, World intellectual property organization, International Publication number WO/2009/144220 A1: Water soluble curcumin compositions for use in anti-cancer and anti-inflammatory therapy. < http://www.wipo.int/patentscope/ search/en/WO2009144220 >, 2009 (accessed 13.07.11).
H. Nojiri, F. Takaku, M. Ohta, Y. Miura, and M. Saito Changes in glycosphingolipid composition during differentiation of human leukemic granulocytes in chronic myelogenous leukemia compared with in vitro granulocytic differentiation of human promyelocytic leukemia cell line HL-60 Cancer Res. 45 1985 6100 6106
P. Nordenfelt, S. Bauer, P. Lonnbro, and H. Tapper Phagocytosis of Streptococcus pyogenes by all-trans retinoic acid-differentiated HL-60 cells: roles of azurophilic granules and NADPH oxidase PLoS One 4 2009
H. Oh, E.R. Mohler, A. Tian, T. Baumgart, and S.L. Diamond Membrane cholesterol is a biomechanical regulator of neutrophil adhesion Arterioscl. Throm. Vas. Biol. 29 2009 1290 1297
J. Pycock, W. Allen, and T. Morris Rapid, single-step isolation of equine neutrophils on a discontinuous Percoll density gradient Res. Vet. Sci. 43 1987 411 412
M. Rama Rao, H. Raghu, and J.S. Rao Regulation of NADPH oxidase (NOX2) by lipid rafts in breast carcinoma cells Editorial Acad. Int. J. Oncol. 37 2010 11
R.C. Reddy, Palakkodu G. Vatsalab, V.G. Keshamounia, and G. Padmanaban Curcumin for malaria therapy Bioche. Biophy. Res. Commun. 326 2005 472 474
S. Reddy, and B.B. Aggarwal Curcumin is a non-competitive and selective inhibitor of phosphorylase kinase FEBS Lett. 341 1994 19 22
C.A. Rice-Evans, N.J. Miller, and G. Paganga Structure-antioxidant activity relationships of flavonoids and phenolic acids Free Rad. Biol. Med. 20 1996 933 956
S. Rungseesantivanon, N. Thenchaisri, P. Ruangvejvorachai, and S. Patumraj Curcumin supplementation could improve diabetes-induced endothelial dysfonction associated with decreased vascular superoxide production and PKC inhibition BioMed. Central Complement. Altern. Medi. 10 2010 48 57
C.C. Sánchez-Martín, A. Dávalos, C. Martín-Sánchez, G. de la Peña, C. Fernández-Hernando, and M.A. Lasunción Cholesterol starvation induces differentiation of human leukemia HL-60 cells Cancer Res. 67 2007 3379 3386
C. Sandersen, D. Oleynik, T. Franck, P. Neven, D. Serteyn, and T. Art Inhalation with NDS27 attenuates pulmonary neutrophilic inflammation in reccurent airway obstruction Vet. Rec. 2011 1 2
N. Sc Non-steroidal anti-inflammatory drugs-uses and complications Singapore Med. J. 33 1992 510 513
F.R. Sheppard, M.R. Kelher, E.E. Moore, N.J.D. McLaughlin, A. Banerjee, and C.C. Silliman Structural organization of the neutrophil NADPH oxidase: phosphorylation and translocation during priming and activation J. Leucocyte Biol. 78 2005 1025 1042
S. Shishodia, G. Sethi, and B.B. Aggarwal Curcumin: getting back to the roots Annal. NY Acad. Sci. 1056 2005 206 217
S. Tamatsu, A.M. Galal, S.A. Ross, D. Ferreira, M.A. ElSohly, A.-R.S. Ibrahim, and F.S. El-Feraly Antioxidant effect of flavonoids on DCF production in HL-60 cells Phytotherapy Res. 17 2003 963 966
J.R. Tennant Evaluation of the trypan blue technique for determination of cell viability Transplantation 2 1964 685 694
O. Teufelhofer, R.-M. Weiss, W. Parzefall, R. Schulte-Hermann, M. Micksche, W. Berger, and L. Elbling Promyelocytic HL-60 cells express NADPH oxidase and are excellent targets in a rapid spectrophotometric microplate assay for extracellular superoxide Toxicol. Sci. 76 2003 376 383
M. Valko, D. Leibfritz, J. Moncola, M.T.D. Cronin, M. Mazura, and J. Telser Free radicals and antioxidants in normal physiological functions and human disease The Int. J. Biochem. Cell Biol. 39 2007 44 84
F. Vilhardt, and B. van Deurs The phagocyte NADPH oxidase depends on cholesterol-enriched membrane microdomains for assembly Eur. Mol. Biol. Organ. J. 23 2004 739 748
M. Yanagida, H. Nakayama, F. Yoshizaki, T. Fujimura, K. Takamori, H. Ogawa, and K. Iwabuchi Proteomic analysis of plasma membrane lipid rafts of HL-60 cells Proteomics 7 2007 2398 2409