Article (Scientific journals)
Effects of eccentrically and concentrically biased training on mouse muscle phenotype
Hody, Stéphanie; Lacrosse, Zoé; Leprince, Pierre et al.
2013In Medicine and Science in Sports and Exercise, 45 (8), p. 1460-1468
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Keywords :
muscle plasticity; muscle fiber typology; mouse muscle proteome; myosin-heavy chains; eccentric contraction
Abstract :
[en] Introduction: The molecular adaptations specifically induced by different muscle contraction types have only been partially elucidated. We previously demonstrated that eccentric contractions in human quadriceps elicited proteome modifications that suggest a muscle fiber typology adaptation. We address this question in a more systematic way by examining here the effects of different running modes on the mouse muscle proteome and the muscle fiber typology. Methods: Male adult mice (C57BL6) were randomly divided into downhill running (DHR, quadricipital eccentrically biased contractions), uphill running (UHR, quadricipital concentrically biased contractions) and untrained control (CONT) groups. Running groups performed five training sessions on an inclined treadmill for 75 to 135 min/day and the quadriceps muscles were dissected 96hours after the last session. Muscle protein extracts of DHR and UHR groups (n=4/group) were subjected to a 2D-DIGE analysis coupled with mass spectrometry. The assessment of fiber type, size and number was performed on the rectus femoris of the three groups (n=6/group) using myosin heavy chain (MHC) immunohistochemistry. Results: In the proteomic analysis, eight spots identified as the fast MHC isoforms exhibited a lower abundance in DHR compared to UHR (p<0.05, t-test). In contrast, ATP synthase subunit α and tubulin β were more expressed in DHR (p<0.05). A significant higher proportion of type I and IIa fibers was found for DHR compared to UHR or CONT groups (p<0.05, one-way ANOVA). Conclusions: Our data suggest that the eccentrically biased contractions in mice induced specific adaptations in protein expression and muscle fiber composition which may reflect a more oxidative muscle phenotype. The differences in stress placed on the muscle between both trainings may be responsible for some unique adaptations resulting from the eccentrically biased training.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Hody, Stéphanie ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Lacrosse, Zoé ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Leprince, Pierre ;  Université de Liège - ULiège > GIGA - Neurosciences
Collodoro, Mike
Croisier, Jean-Louis ;  Université de Liège - ULiège > Département des sciences de la motricité > Kinésithérapie générale et réadaptation
Rogister, Bernard  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Language :
English
Title :
Effects of eccentrically and concentrically biased training on mouse muscle phenotype
Publication date :
August 2013
Journal title :
Medicine and Science in Sports and Exercise
ISSN :
0195-9131
eISSN :
1530-0315
Publisher :
Lippincott Williams & Wilkins, United States - Maryland
Volume :
45
Issue :
8
Pages :
1460-1468
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 January 2013

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