Article (Scientific journals)
Fast-GC quantifacation of harmonine, the major defense alkaloid of the multicolored Asian ladybird Harmonia axyridis (Pallas)
Fischer, Christophe; Laurent, Pascal; Heuskin, Stéphanie et al.
2012In American Journal of Analytical Chemistry, 3, p. 65-75
Peer reviewed
 

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Keywords :
alkaloid; harmonine; Harmonia axyridis; Fast-GC; validation
Abstract :
[en] These last few years the multicolored Asian ladybird, Harmonia axyridis (Pallas), previously introduced in Europe and North America as a biological control agent, has swiftly spread out on those territories and turns out to be a pest in several ways. In order to improve understanding of several key points of the biology of this ladybird, a novel fast-GC method of quantification of harmonine, the main defense alkaloid of H. axyridis, has been designed and validated using the accuracy profile concept for concentrations ranging from 13 to 264 µg/individual. This method allows quantifying harmonine in a single insect with a maximal error risk of 20%. For ladybirds collected in spring, mean harmonine concentration observed was 106.6 µg/individual with a very high standard deviation of 80.2 µg/individual, which can easily be explained by the strong asymmetry of the results distribution. Males and females seem equally chemically protected as the harmonine concentration did not significantly differ between sexes. This method is currently used to improve our comprehension of several key points of H. axyridis biology including aggregation behavior and aposematism.
Disciplines :
Entomology & pest control
Chemistry
Author, co-author :
Fischer, Christophe ;  Université de Liège - ULiège > Chimie et bio-industries > Analyse, qual. et risques - Labo. de Chimie analytique
Laurent, Pascal ;  Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Heuskin, Stéphanie ;  Université de Liège - ULiège > Chimie et bio-industries > Analyse, qual. et risques - Labo. de Chimie analytique
Durieux, Delphine ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Haubruge, Eric  ;  Université de Liège - ULiège > Services administratifs généraux > Vice-Recteur de Gembloux Agro Bio Tech
Verheggen, François  ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Lognay, Georges ;  Université de Liège - ULiège > Chimie et bio-industries > Analyse, qual. et risques - Labo. de Chimie analytique
Language :
English
Title :
Fast-GC quantifacation of harmonine, the major defense alkaloid of the multicolored Asian ladybird Harmonia axyridis (Pallas)
Publication date :
January 2012
Journal title :
American Journal of Analytical Chemistry
ISSN :
2156-8251
eISSN :
2156-8278
Publisher :
Scientific Research Publishing, Irvine, United States - California
Volume :
3
Pages :
65-75
Peer reviewed :
Peer reviewed
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
Available on ORBi :
since 14 September 2012

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