Article (Scientific journals)
An alternative role of FoF1-ATP synthase in Escherichia coli: synthesis of thiamine triphosphate
Gigliobianco, Tiziana; GANGOLF, Marjorie; Lakaye, Bernard et al.
2013In Scientific Reports, 3 (1071)
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Keywords :
Thiamine; Chemiosmotic mechanism; ATP synthase; energy metabolism; amino acid starvation; FoF1-ATP synthase
Abstract :
[en] In E. coli, thiamine triphosphate (ThTP), a putative signaling molecule, transiently accumulates in response to amino acid starvation. This accumulation requires the presence of an energy substrate yielding pyruvate. Here we show that in intact bacteria ThTP is synthesized from free thiamine diphosphate (ThDP) and Pi, the reaction being energized by the proton-motive force (Dp) generated by the respiratory chain. ThTP production is suppressed in strains carrying mutations in F1 or a deletion of the atp operon. Transformation with a plasmid encoding the whole atp operon fully restored ThTP production, highlighting the requirement for FoF1-ATP synthase in ThTP synthesis. Our results show that, under specific conditions of nutritional downshift, FoF1-ATP synthase catalyzes the synthesis of ThTP, rather than ATP, through a highly regulated process requiring pyruvate oxidation. Moreover, this chemiosmotic mechanism for ThTP production is conserved from E. coli to mammalian brain mitochondria.
Research center :
Giga-Neurosciences - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Gigliobianco, Tiziana ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
GANGOLF, Marjorie ;  Centre Hospitalier Universitaire de Liège - CHU > Service d'Informations médico économiques (SIME)
Lakaye, Bernard ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique
Pirson, Bastien
von Ballmoos, Christoph
Wins, Pierre
Bettendorff, Lucien  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique
Language :
English
Title :
An alternative role of FoF1-ATP synthase in Escherichia coli: synthesis of thiamine triphosphate
Publication date :
January 2013
Journal title :
Scientific Reports
eISSN :
2045-2322
Publisher :
Nature Publishing group
Volume :
3
Issue :
1071
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRFC - Fonds de la Recherche Fondamentale Collective [BE]
SNSF - Swiss National Science Foundation [CH]
Sverige Vetenskapsrådet [SE]
Available on ORBi :
since 16 January 2013

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