Reference : Targeting steroid receptor coactivator-1 expression with locked nucleic acids antisen...
Scientific journals : Article
Social & behavioral sciences, psychology : Neurosciences & behavior
http://hdl.handle.net/2268/13889
Targeting steroid receptor coactivator-1 expression with locked nucleic acids antisense reveals different thresholds for the hormonal regulation of male sexual behavior in relation to aromatase activity and protein expression.
English
Charlier, Thierry mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau >]
Harada, Nobuhiro [> > > >]
Ball, Gregory F [> > > >]
Balthazart, Jacques mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau >]
2006
Behavioural Brain Research
Elsevier Science
172
2
333-43
Yes (verified by ORBi)
International
0166-4328
Amsterdam
The Netherlands
[en] Analysis of Variance ; Animals ; Aromatase/metabolism ; Coturnix ; Gene Expression Regulation ; Histone Acetyltransferases/genetics/metabolism ; Hypothalamus/enzymology ; Injections, Intraventricular ; Male ; Neuronal Plasticity/physiology ; Oligonucleotides ; Oligonucleotides, Antisense/administration & dosage ; Preoptic Area/enzymology ; Receptors, Androgen/metabolism ; Receptors, Estrogen/metabolism ; Sexual Behavior, Animal/physiology ; Signal Transduction/physiology ; Statistics, Nonparametric ; Testosterone/metabolism ; Time Factors ; Transcription Factors/genetics/metabolism
[en] Steroid receptors such as the androgen and estrogen receptors require the presence of several proteins, known as coactivators, to enhance the transcription of target genes. The first goal of the present study was to define the role of SRC-1 on the steroid-dependent expression of the aromatase protein and its activity in male Japanese quail. The second goal was to analyze the rapid plasticity of the POM following antisense treatment interruption. We confirm here that the inhibition of SRC-1 expression by daily intracerebroventricular injections of locked nucleic acid antisense oligonucleotides in the third ventricle at the level of the preoptic area-hypothalamus (HPOA) significantly reduces testosterone-dependent male sexual behavior. In the first experiment, aromatase protein expression in HPOA was inhibited in SRC-1-depleted males but the enzymatic activity remained at the level measured in controls. We observed in the second experiment a recovery of the behavioral response to testosterone treatment after interruption of the antisense injection. However, several morphological characteristics of the POM were not different between the control group, the antisense-treated birds and antisense-treated birds in which treatment had been discontinued 3 days earlier. Antisense was also less effective in knocking-down SRC-1 in the present experiments as compared to our previous study. An analysis of this variation in the degree of knock-down of SRC-1 expression suggests dissociation among different aspects of steroid action on brain and behavior presumably resulting from the differential sensitivity of behavioral and neurochemical responses to the activation by testosterone and/or its estrogenic metabolites.
http://hdl.handle.net/2268/13889
also: http://hdl.handle.net/2268/11035
10.1016/j.bbr.2006.05.023

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Papier 13 LNABehav Brain Res.pdfNo commentaryPublisher postprint293.3 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.