ORBi: Lansiaux A. - Apoptosis of HL-60 leukemia cells induced by the bisindole alkaloids sungucine and isosungucine from Strychnos icaja

Reference : Apoptosis of HL-60 leukemia cells induced by the bisindole alkaloids sungucine and isosu...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Phytobiology (plant sciences, forestry, mycology...) Physical, chemical, mathematical & earth Sciences : Chemistry Human health sciences : Pharmacy, pharmacology & toxicology
	To cite this reference:	http://hdl.handle.net/2268/13885

Title :	Apoptosis of HL-60 leukemia cells induced by the bisindole alkaloids sungucine and isosungucine from Strychnos icaja
Language :	English
Author, co-author :	Lansiaux, A. [> > > >]
	Bailly, Claude [Université de Liège - ULg > Ressources immobilières > ARI : Cellule accueil du bâtiment L3 >]
	Houssier, Claude [Université de Liège - ULg > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences) >]
	Colson, Pierre [Université de Liège - ULg > > Chimie physique biologique >]
	Gillet, Marie-Claire [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques >]
	Frederich, Michel [Université de Liège - ULg > Département de pharmacie > Pharmacognosie >]
	Tits, Monique [Université de Liège - ULg > Département de pharmacie > Phytochimie et phytothérapie >]
	Angenot, Luc [Université de Liège - ULg > Département de pharmacie > Pharmacognosie >]
Publication date :	Jul-2002
Journal title :	Planta Medica
Publisher :	Georg Thieme Verlag Kg
Volume :	68
Issue/season :	7
Pages :	591-595
Audience :	International
ISSN :	0032-0943
City :	Stuttgart
Keywords :	[en] sungucine ; isosungucine ; plant alkaloids ; DNA binding ; DNA synthesis ; apoptosis ; Strychnos icaja ; Loganiaceae
Abstract :	[en] Sungucine and isosungucine are two bisindole alkaloids isolated from the roots of the African plant Strychnos icaja Baillon. They both exhibit antiplasmodial activities but also show cytotoxic effects against human cancer cell lines. In order to elucidate their mechanism of action, we have investigated the interaction of the alkaloids with DNA and their capacity to inhibit nucleic acids and protein synthesis in the human HL-60 promyelocytic leukemia cell line. Cell treatment with both sungucine and isosungucine leads to the appearance of a hypo-diploid DNA content peak. Western blotting analysis reveals that the two alkaloids induce cleavage of the poly(ADP-ribose) polymerase (PARP) and promote the cleavage of a caspase-3 DEVD peptide substrate. The activation of the caspase cascade is accompanied with a fragmentation of DNA in cells, as revealed by the TUNEL assay. Altogether, the results shed light on the mechanism of action of these two plant alkaloids and identify signaling factors involved in (iso)sungucine-induced apoptosis in HL-60 cells.
Permalink :	http://hdl.handle.net/2268/13885

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