Effect of omp10 or omp19 deletion on Brucella abortus outer membrane properties and virulence in mice.

Animals; Antigens, Bacterial; Bacterial Outer Membrane Proteins/genetics/physiology; Brucella abortus/genetics/growth & development/pathogenicity/physiology; Brucellosis/etiology/microbiology; Cattle; Cell Line; Female; Gene Deletion; Genes, Bacterial; Hela Cells; Humans; Lipoproteins; Mice; Mice, Inbred BALB C; Mutation

Abstract :

[en] The distinctive properties of Brucella outer membrane have been considered to be critical for Brucella sp. virulence. Among the outer membrane molecules possibly related to these properties, Omp10 and Omp19 are immunoreactive outer membrane lipoproteins. Moreover, these proteins of Brucella could constitute a new family of outer membrane proteins specifically encountered in the family RHIZOBIACEAE: We evaluated the impact of omp10 or omp19 deletion on Brucella abortus outer membrane properties and virulence in mice. The omp10 mutant was dramatically attenuated for survival in mice and was defective for growth in minimal medium but was not impaired in intracellular growth in vitro, nor does it display clear modification of the outer membrane properties. Significantly fewer brucellae were recovered from the spleens of mice infected with the omp19 mutant than from those of mice infected with the parent strain at 4 and 8 weeks postinfection. The omp19 mutant exhibited an increase in sensitivity to the polycation polymyxin B and to sodium deoxycholate. These results indicate that inactivation of the omp19 gene alters the outer membrane properties of B. abortus.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Tibor, Anne

Wansard, Valerie

Bielartz, Valery

Delrue, Rose-May

DANESE, Isabelle ; Université de Liège - ULiège > GIGA-M : Coordination scientifique

Michel, Patrick

Walravens, Karl

Godfroid, Jacques

Letesson, Jean-Jacques

Language :

English

Title :

Effect of omp10 or omp19 deletion on Brucella abortus outer membrane properties and virulence in mice.

Publication date :

2002

Journal title :

Infection and Immunity

ISSN :

0019-9567

eISSN :

1098-5522

Publisher :

American Society for Microbiology, Washington, United States - District of Columbia

Volume :

Issue :

Pages :

5540-6

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 10 January 2013

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