Article (Scientific journals)
Quantitative proteomic analysis to decipher the differential apoptotic response of bortezomib-treated APL cells before and after retinoic acid differentiation reveals involvement of protein toxicity mechanisms.
Uttenweiler-Joseph, Sandrine; Bouyssié, David; Calligaris, David et al.
2013In Proteomics, 13, p. 37-47
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Keywords :
Cell biology; iTRAQ; Kinetic of protein variation; Proteasome inhibition
Abstract :
[en] The ubiquitin-proteasome system allows the targeted degradation of proteins and plays a critical role in the regulation of many cellular processes. Proteasome inhibition is a recent antitumor therapeutic strategy and bortezomib was the first proteasome inhibitor approved for clinical use. In this study, we used the NB4 cell line to investigate the effects of bortezomib toward acute promyelocytic leukemia cells before and after retinoic acid-induced differentiation. We showed that apoptosis level after bortezomib treatment is higher in NB4 cells than in differentiated NB4 cells. To compare early protein variations upon bortezomib treatment in both NB4 cell populations, we performed a quantitative proteomic analysis based on iTRAQ peptide labeling followed by data analysis with in-house developed scripts. This strategy revealed the regulation of 14 proteins principally involved in protein stress response and apoptosis in NB4 cells after proteasome inhibition. Altogether, our results suggest that the differential level of apoptosis induced by bortezomib treatment in both NB4 cell populations could result from distinct protein toxicity level.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Uttenweiler-Joseph, Sandrine;  Universite de Toulouse, UPS, IPBS, Toulouse, France > CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), Toulouse, France
Bouyssié, David;  Universite de Toulouse, UPS, IPBS, Toulouse, France > CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), Toulouse, France
Calligaris, David ;  Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Lutz, Pierre G.;  Universite de Toulouse, UPS, IPBS, Toulouse, France > CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), Toulouse, France
Monsarrat, Bernard;  Universite de Toulouse, UPS, IPBS, Toulouse, France > CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), Toulouse, France
Burlet-Schiltz, Odile;  Universite de Toulouse, UPS, IPBS, Toulouse, France > CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), Toulouse, France
Language :
English
Title :
Quantitative proteomic analysis to decipher the differential apoptotic response of bortezomib-treated APL cells before and after retinoic acid differentiation reveals involvement of protein toxicity mechanisms.
Publication date :
2013
Journal title :
Proteomics
ISSN :
1615-9853
eISSN :
1615-9861
Publisher :
Wiley-VCH Verlag Gmbh, Weinheim, Germany
Volume :
13
Pages :
37-47
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 07 January 2013

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