Article (Scientific journals)
ORF9p phosphorylation by ORF47p is crucial for the formation and egress of the Varicella-zoster virus (VZV) viral particles.
Riva, Laura; Thiry, Marc; BONTEMS, Sébastien et al.
2013In Journal of Virology, 87 (5), p. 2868-2881
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Keywords :
Varicella Zoster virus; ORF9p; phosphorylation
Abstract :
[en] The role of the tegument during the herpesvirus lytic cycle is still not clearly established, particularly at the late phase of infection, when the newly produced viral particles need to be fully assembled before being released from the infected cell. The Varicella-zoster virus (VZV) protein coded by ORF9 (ORF9p) is an essential tegument protein and, even though its mRNA is the most expressed during the productive infection, little is known about its functions. Using a GalK positive/negative selection technique, we modified a BAC containing the complete VZV genome creating viruses expressing mutant versions of ORF9p.We showed that ORF9p is hyper-phosphorylated during the infection, especially through its interaction with the viral Ser/Thr kinase ORF47p; we identified a consensus site within ORF9p recognized by ORF47p and demonstrated its importance for ORF9p phosphorylation. Strikingly, an ultra-structural analysis revealed that the mutation of this consensus site (Glutamate 85 to Arginine) strongly affects viral assembly and release, reproducing ORF47 kinase dead VZV phenotype. It also slightly diminishes the infectivity towards immature dendritic cells. Taken together, our results identify ORF9p as a new viral substrate of ORF47p and suggest a determinant role of this phosphorylation for viral infectivity, especially during the process of viral particle formation and egress.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Riva, Laura ;  Université de Liège - ULiège > GIGA-R : Virologie - Immunologie
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
BONTEMS, Sébastien ;  Centre Hospitalier Universitaire de Liège - CHU
Joris, Aline ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie - Immunologie
Piette, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie - Immunologie
Lebrun, Marielle   ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie et immunologie
Sadzot-Delvaux, Catherine  ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie et immunologie
 These authors have contributed equally to this work.
Language :
English
Title :
ORF9p phosphorylation by ORF47p is crucial for the formation and egress of the Varicella-zoster virus (VZV) viral particles.
Publication date :
2013
Journal title :
Journal of Virology
ISSN :
0022-538X
eISSN :
1098-5514
Publisher :
American Society for Microbiology, Washington, United States - District of Columbia
Volume :
87
Issue :
5
Pages :
2868-2881
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 07 January 2013

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