Article (Scientific journals)
Glycosyl transferase activity of the Escherichia coli penicillin-binding protein 1b: Specificity profile for the substrate
Fraipont, Claudine; Sapunaric, Frédéric; Zervosen, Astrid et al.
2006In Biochemistry, 45 (12), p. 4007-4013
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Keywords :
glycosyl-transferase, lipid II
Abstract :
[en] The glycosyl transferase of the Escherichia coli bifunctional penicillin-binding protein (PBP) 1b catalyzes the assembly of lipid-transported N-acetylglucosaminyl-beta-1,4-N-acetylmuramoyl-L-Ala-gamma-D-Glu-meso-A(2)pm-D-Ala-D-Ala units (lipid II) into linear peptidoglycan chains. These units are linked, at C1 of N-acetylmuramic acid (MurNAc), to a C-55 undecaprenyl pyrophosphate. In an in vitro assay, lipid II functions both as a glycosyl donor and as a glycosyl acceptor substrate. Using substrate analogues, it is suggested that the specificity of the enzyme for the glycosyl donor substrate differs from that for the acceptor. The donor substrate requires the presence of both N-acetylglucosamine (GlcNAc) and MurNAc and a reactive group on C1 of the MurNAc and does not absolutely require the lipid chain which can be replaced by uridine. The enzyme appears to prefer an acceptor substrate containing a polyprenyl pyrophosphate on C1 of the MurNAc sugar. The problem of glycan chain elongation that presumably proceeds by the repetitive addition of disaccharide peptide units at their reducing end is discussed.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Fraipont, Claudine ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Sapunaric, Frédéric ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Zervosen, Astrid ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Auger, Geneviève;  Université Paris-Sud 11
Devreese, Bart;  Rijksuniversiteit Gent
Lioux, Thierry;  Katholieke Universiteit Leuven - KUL
Blanot, Didier;  Université Paris-Sud 11
Mengin-Lecreulx, Dominique;  Université Paris-Sud 11
Herdewijn, Piet;  Katholieke Universiteit Leuven - KUL
Van Beeumen, Jos;  Rijksuniversiteit Gent
Frère, Jean-Marie ;  Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie
Nguyen-Distèche, Martine ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Language :
English
Title :
Glycosyl transferase activity of the Escherichia coli penicillin-binding protein 1b: Specificity profile for the substrate
Publication date :
28 March 2006
Journal title :
Biochemistry
ISSN :
0006-2960
eISSN :
1520-4995
Publisher :
Amer Chemical Soc, Washington, United States - Washington
Volume :
45
Issue :
12
Pages :
4007-4013
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 June 2009

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