Article (Scientific journals)
Large-scale identification of selenium metabolites by online size-exclusion-reversed phase liquid chromatography with combined inductively coupled plasma (ICP-MS) and electrospray ionization linear trap-Orbitrap mass spectrometry (ESI-MS(n))
Preudhomme, Hugues; Far, Johann; Gil-Casal, Sandra et al.
2012In Metallomics, 4 (5), p. 422-432
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Abstract :
[en] A method was developed for the comprehensive cartography of the selenium metabolites synthesized in the process of conversion of selenite [Se(IV)] into organic compounds and enrichment of yeast with selenium. The number of compounds detected was considerably increased (49) owing to the optimization of the fractionation procedure and the use of UPLC. The increased purity of the minor selenoorganic amino acids and oligopeptides allowed successful on-line de-novo identification based on the exact mass (o1 ppm) and fragmentation (MS2/MS3) mass spectra obtained with high resolution and high mass accuracy. The quality of mass spectra allowed the re-interpretation of previously reported structures of some selenium species.
Disciplines :
Chemistry
Author, co-author :
Preudhomme, Hugues;  CNRS/UPPA Laboratoire de Chimie Analytique BioInorganique et Environnement UMR5254 Helioparc 2av P Angot PAU France > UMR5254
Far, Johann  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Gil-Casal, Sandra;  CNRS/UPPA Laboratoire de Chimie Analytique BioInorganique et Environnement UMR5254 Helioparc 2av P Angot PAU France
Lobinsky, Ryzsard;  CNRS/UPPA Laboratoire de Chimie Analytique BioInorganique et Environnement UMR5254 Helioparc 2av P Angot PAU France
Language :
English
Title :
Large-scale identification of selenium metabolites by online size-exclusion-reversed phase liquid chromatography with combined inductively coupled plasma (ICP-MS) and electrospray ionization linear trap-Orbitrap mass spectrometry (ESI-MS(n))
Publication date :
May 2012
Journal title :
Metallomics
ISSN :
1756-591X
eISSN :
1756-5901
Publisher :
Royal Society of Chemistry
Volume :
4
Issue :
5
Pages :
422-432
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 16 December 2012

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