Reference : State-of-the-art methodologies for the discovery and characterization of DNA G-quadru...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences : Chemistry
Human health sciences : Pharmacy, pharmacology & toxicology
State-of-the-art methodologies for the discovery and characterization of DNA G-quadruplex binders.
Pagano, Bruno [> >]
Cosconati, Sandro [> >]
Gabelica, Valérie mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Petraccone, Luigi [> >]
De Tito, Stefano [> >]
Marinelli, Luciana [> >]
La Pietra, Valeria [> >]
di Leva, Francesco Saverio [> >]
Lauri, Ilaria [> >]
Trotta, Roberta [> >]
Novellino, Ettore [> >]
Giancola, Concetta [> >]
Randazzo, Antonio [> >]
Current Pharmaceutical Design
Yes (verified by ORBi)
[en] Calorimetry ; Circular Dichroism ; DNA/chemistry ; Drug Discovery ; Fluorescence Resonance Energy Transfer ; G-Quadruplexes ; Humans ; Ligands ; Magnetic Resonance Spectroscopy ; Spectrometry, Fluorescence ; Spectrometry, Mass, Electrospray Ionization ; review
[en] Nowadays, the molecular basis of interaction between low molecular weight compounds and biological macromolecules is the subject of numerous investigations aimed at the rational design of molecules with specific therapeutic applications. In the last decades, it has been demonstrated that DNA quadruplexes play a critical role in several biological processes both at telomeric and gene promoting levels thus providing a great stride in the discovery of ligands able to interact with such a biologically relevant DNA conformation. So far, a number of experimental and computational approaches have been successfully employed in order to identify new ligands and to characterize their binding to the DNA. The main focus of this review is the description of these methodologies, placing a particular emphasis on computational methods, isothermal titration calorimetry (ITC), mass spectrometry (MS), nuclear magnetic resonance (NMR), circular dichroism (CD) and fluorescence spectroscopies.
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