Article (Scientific journals)
Effects of glycerol on Pseudomonas fluorescens BTP1 freeze-dried
Mputu Kanyinda, Jean-Noël; Pierart, C.; Weekers, F. et al.
2012In International Journal of Biotechnology and Biochemistry, 8 (2), p. 245-258
 

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Keywords :
Glutathione; cellular fatty acids; Proteins; Bio-fungicide; Bio-fongicide
Abstract :
[en] The storage stability of freeze-dried powders was studied by parameters such as loss of viability on the Plate Count Agar (PCA). Powder with glycerol (PG) contains 8.4x1010cfu/g before storage 1.1x1010cfug after 3 months at 4°C and 6.0x108cfu/g after 3 months at 20°C. The concentration of soluble proteins (mg/g) decrease during storage at 4°C from 3.77 to 0.80 after 90 days; and the ratios of unsaturated to saturated fatty acids (C18:3/C16:0 and C18:2/C16:0) decrease respectively from 0.05 to 0.04 and 0.007 to 0.004 after 3 months at 4°C. This ratio characterises the membrane fluidity. Powder without glycerol (PS) contains 1.1x1010 cfu/g before storage and 1.4 x 108 cfu/g after 3 months at 4°C and 1.4 x 107 cfu/g after 3 months at 20°C. The concentration of soluble proteins (mg/g) decrease during storage at 4°C from 4.08 to 0.42 after 90 days, the glutathione concentration decrease during storage at 4°C from 2.2 to 1.4. The beneficial effect of glycerol on fatty acid composition during freezedrying is shown and the ratios of unsaturated to saturated fatty acids (C18:2/C16:0 and C18:3/C16:0) decrease respectively from 0.019 to 0.004 and 0.054 to 0.036 after 90 days storage at 4°C. Analysis by flow cytometry was used to assess the physiological state in which cells are at the end of freeze-drying. We found 13.5% live cells, 36.1% dead cells and 50.4% cells in an intermediate state for powder with glycerol (PG) after freeze-drying. These results shows that glycerol play an important role in Pseudomonas fluorescens BTP1 desiccation during freeze-drying, by maintaining a degree of viability after freeze-drying and during storage.
Research center :
Centre Wallon de Biologie Industrielle
Disciplines :
Biotechnology
Author, co-author :
Mputu Kanyinda, Jean-Noël ;  Université de Liège - ULiège > Doct. sc. agro. & ingé. biol.
Pierart, C.
Weekers, F.
Delvigne, Frank  ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Destain, Jacqueline ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Wathelet, Jean-Paul ;  Université de Liège - ULiège > Chimie et bio-industries > Chimie générale et organique
Thonart, Philippe ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Language :
English
Title :
Effects of glycerol on Pseudomonas fluorescens BTP1 freeze-dried
Publication date :
24 September 2012
Journal title :
International Journal of Biotechnology and Biochemistry
ISSN :
0973-2691
eISSN :
0974-4762
Publisher :
Research India Publications, Delhi, India
Volume :
8
Issue :
2
Pages :
245-258
Name of the research project :
Conseil Alimentaire (CONSALIM)
Funders :
CTB and CONSALIM
Available on ORBi :
since 06 December 2012

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