Reference : Effects of glycerol on Pseudomonas fluorescens BTP1 freeze-dried
Scientific journals : Article
Life sciences : Biotechnology
http://hdl.handle.net/2268/135835
Effects of glycerol on Pseudomonas fluorescens BTP1 freeze-dried
English
Mputu Kanyinda, Jean-Noël mailto [Université de Liège - ULg > > > Doct. sc. agro. & ingé. biol.]
Pierart, C. []
Weekers, F. []
Delvigne, Frank mailto [Université de Liège - ULg > Chimie et bio-industries > Bio-industries >]
Destain, Jacqueline mailto [Université de Liège - ULg > Chimie et bio-industries > Bio-industries >]
Wathelet, Jean-Paul mailto [Université de Liège - ULg > Chimie et bio-industries > Chimie générale et organique >]
Thonart, Philippe mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
24-Sep-2012
International Journal of Biotechnology and Biochemistry.
Research India Publications
8
2
245-258
No
International
0973-2691
Delhi
India
[en] Glutathione ; cellular fatty acids ; Proteins ; Bio-fungicide ; Bio-fongicide
[en] The storage stability of freeze-dried powders was studied by parameters such
as loss of viability on the Plate Count Agar (PCA). Powder with glycerol (PG)
contains 8.4x1010cfu/g before storage 1.1x1010cfug after 3 months at 4°C and
6.0x108cfu/g after 3 months at 20°C. The concentration of soluble proteins
(mg/g) decrease during storage at 4°C from 3.77 to 0.80 after 90 days; and the
ratios of unsaturated to saturated fatty acids (C18:3/C16:0 and C18:2/C16:0)
decrease respectively from 0.05 to 0.04 and 0.007 to 0.004 after 3 months at
4°C. This ratio characterises the membrane fluidity. Powder without glycerol
(PS) contains 1.1x1010 cfu/g before storage and 1.4 x 108 cfu/g after 3 months
at 4°C and 1.4 x 107 cfu/g after 3 months at 20°C. The concentration of soluble
proteins (mg/g) decrease during storage at 4°C from 4.08 to 0.42 after 90 days,
the glutathione concentration decrease during storage at 4°C from 2.2 to 1.4.
The beneficial effect of glycerol on fatty acid composition during freezedrying
is shown and the ratios of unsaturated to saturated fatty acids
(C18:2/C16:0 and C18:3/C16:0) decrease respectively from 0.019 to 0.004
and 0.054 to 0.036 after 90 days storage at 4°C. Analysis by flow cytometry
was used to assess the physiological state in which cells are at the end of
freeze-drying. We found 13.5% live cells, 36.1% dead cells and 50.4% cells in an intermediate state for powder with glycerol (PG) after freeze-drying. These
results shows that glycerol play an important role in Pseudomonas fluorescens
BTP1 desiccation during freeze-drying, by maintaining a degree of viability
after freeze-drying and during storage.
Centre Wallon de Biologie Industrielle
CTB and CONSALIM
Conseil Alimentaire (CONSALIM)
Researchers ; Students ; General public
http://hdl.handle.net/2268/135835

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