ORBi: Mputu Kanyinda Jean-Noël - Effects of glycerol on Pseudomonas fluorescens BTP1 freeze-dried

Reference : Effects of glycerol on Pseudomonas fluorescens BTP1 freeze-dried


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biotechnology
	To cite this reference:	http://hdl.handle.net/2268/135835

Title :	Effects of glycerol on Pseudomonas fluorescens BTP1 freeze-dried
Language :	English
Author, co-author :	Mputu Kanyinda, Jean-Noël [Université de Liège - ULg > > > Doct. sc. agro. & ingé. biol.]
	Pierart, C. []
	Weekers, F. []
	Delvigne, Frank [Université de Liège - ULg > Chimie et bio-industries > Bio-industries >]
	Destain, Jacqueline [Université de Liège - ULg > Chimie et bio-industries > Bio-industries >]
	Wathelet, Jean-Paul [Université de Liège - ULg > Chimie et bio-industries > Chimie générale et organique >]
	Thonart, Philippe [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Publication date :	24-Sep-2012
Journal title :	International Journal of Biotechnology and Biochemistry.
Publisher :	Research India Publications
Volume :	8
Issue/season :	2
Pages :	245-258
Peer reviewed :	No
Audience :	International
ISSN :	0973-2691
City :	Delhi
Country :	India
Keywords :	[en] Glutathione ; cellular fatty acids ; Proteins ; Bio-fungicide ; Bio-fongicide
Abstract :	[en] The storage stability of freeze-dried powders was studied by parameters such as loss of viability on the Plate Count Agar (PCA). Powder with glycerol (PG) contains 8.4x1010cfu/g before storage 1.1x1010cfug after 3 months at 4°C and 6.0x108cfu/g after 3 months at 20°C. The concentration of soluble proteins (mg/g) decrease during storage at 4°C from 3.77 to 0.80 after 90 days; and the ratios of unsaturated to saturated fatty acids (C18:3/C16:0 and C18:2/C16:0) decrease respectively from 0.05 to 0.04 and 0.007 to 0.004 after 3 months at 4°C. This ratio characterises the membrane fluidity. Powder without glycerol (PS) contains 1.1x1010 cfu/g before storage and 1.4 x 108 cfu/g after 3 months at 4°C and 1.4 x 107 cfu/g after 3 months at 20°C. The concentration of soluble proteins (mg/g) decrease during storage at 4°C from 4.08 to 0.42 after 90 days, the glutathione concentration decrease during storage at 4°C from 2.2 to 1.4. The beneficial effect of glycerol on fatty acid composition during freezedrying is shown and the ratios of unsaturated to saturated fatty acids (C18:2/C16:0 and C18:3/C16:0) decrease respectively from 0.019 to 0.004 and 0.054 to 0.036 after 90 days storage at 4°C. Analysis by flow cytometry was used to assess the physiological state in which cells are at the end of freeze-drying. We found 13.5% live cells, 36.1% dead cells and 50.4% cells in an intermediate state for powder with glycerol (PG) after freeze-drying. These results shows that glycerol play an important role in Pseudomonas fluorescens BTP1 desiccation during freeze-drying, by maintaining a degree of viability after freeze-drying and during storage.
Research units :	Centre Wallon de Biologie Industrielle
Funders :	CTB and CONSALIM
Name of the research project :	Conseil Alimentaire (CONSALIM)
Target :	Researchers ; Students ; General public
Permalink :	http://hdl.handle.net/2268/135835

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