ORBi: Mputu Kanyinda Jean-Noël - Impact of glycerol and storage temperature on gluatathione concentration and physiological state of Pseudomonas fluorescens BTP1 freeze-dried

Reference : Impact of glycerol and storage temperature on gluatathione concentration and physiologic...


	Document type :	Scientific congresses and symposiums : Poster
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/135834

Title :	Impact of glycerol and storage temperature on gluatathione concentration and physiological state of Pseudomonas fluorescens BTP1 freeze-dried
Language :	English
Author, co-author :	Mputu Kanyinda, Jean-Noël [Université de Liège - ULg > > > Doct. sc. agro. & ingé. biol.]
	Pierart, C. []
	Delvigne, Frank [Université de Liège - ULg > Chimie et bio-industries > Bio-industries >]
	Destain, Jacqueline [Université de Liège - ULg > Chimie et bio-industries > Bio-industries >]
	Thonart, Philippe [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Publication date :	15-Feb-2012
Page number :	2x1m
On invitation :	Yes
Audience :	National
Event name :	17th Symposium on Applied Biological Sciences
Event date :	15-2-2012
Event organizer :	KU Leuven
Event place (city) :	Leuven
Event country :	Belgium
Keywords :	[en] Glutathione ; Freeze-drying ; Proteins
Abstract :	[en] Pseudomonas fluorescens is commonly used as bio-fungicides in agriculture. For this use it requires formulations as either liquid or powder. Formulations have two advantages, storage and transport. Freeze-drying is a commonly used method to preserve bacteria. However, freeze-drying damages the cells, which results in loss of viability. Protective compounds are used to reduce loss of viability during process (freeze-drying and storage). In our study we used flow cytometry analysis to assess the physiological state in which cells are at the end of freeze-drying and Glutathione (GSH) was measured before and during storage.
Research units :	Centre Wallon de Biologie Industrielle
Funders :	CONSALIM Project of Walonia
Name of the research project :	Conseil Alimentaire (CONSALIM)
Target :	Students
Permalink :	http://hdl.handle.net/2268/135834

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