Thonart, Philippe[Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
17th Symposium on Applied Biological Sciences
[en] Glutathione ; Freeze-drying ; Proteins
[en] Pseudomonas fluorescens is commonly used as bio-fungicides in agriculture. For this use it requires formulations as either liquid or powder. Formulations have two advantages, storage and transport. Freeze-drying is a commonly used method to preserve bacteria. However, freeze-drying damages the cells, which results in loss of viability. Protective compounds are used to reduce loss of viability during process (freeze-drying and storage). In our study we used flow cytometry analysis to assess the physiological state in which cells are at the end of freeze-drying and Glutathione (GSH) was measured before and during storage.