ORBi: Garbacki Nancy - Effects of prodelphinidins isolated from Ribes nigrum on chondrocyte metabolism and COX activity

Reference : Effects of prodelphinidins isolated from Ribes nigrum on chondrocyte metabolism and COX ...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Pharmacy, pharmacology & toxicology
	To cite this reference:	http://hdl.handle.net/2268/13573

Title :	Effects of prodelphinidins isolated from Ribes nigrum on chondrocyte metabolism and COX activity
Language :	English
Author, co-author :	Garbacki, Nancy [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Protéines et glycoprot. de matr.extracell. et membran.basal. >]
	Angenot, Luc [Université de Liège - ULg > Département de pharmacie > Pharmacognosie >]
	Bassleer, C. [> > > >]
	Damas, Jacques [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
	Tits, Monique [Université de Liège - ULg > Département de pharmacie > Phytochimie et phytothérapie >]
Publication date :	Jun-2002
Journal title :	Naunyn-Schmiedeberg's Archives of Pharmacology
Publisher :	Springer-Verlag
Volume :	365
Issue/season :	6
Pages :	434-441
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0028-1298
City :	New York
Keywords :	[en] Ribes nigrum ; prodelphinidins ; osteoarthritis ; cyclooxygenases ; chondrocytes
Abstract :	[en] Articular diseases. such as osteoarthritis, is the clinical expression of the loss of cartilage function. COX inhibitors are widely used in the treatment of such pathologies for their beneficial effects on inflammation but often produce a negative activity on cartilage synthesis. In this study, we determined the effect of different prodelphinidins, the major compounds isolated from Ribes nigrum leaves, on the proteoglycans (PGs), type II collagen (coll. II) and prostaglandin E-2 (PGE(2)) production by differentiated human chondrocytes cultivated in long term (12 days) and in clusters as well as their inhibition potential on COX-1 and COX-2 in vitro. Gallocatechin trimer (GC-GC-GC) showed the higher stimulation of PGs and coll. II production (1 mug ml(-1)) and the synthesis of PGE(2) was significantly reduced by gallocatechin dimer (GC-GC), gallocatechin-epigallocatechin (GC-EGC) and GC-GC-GC at 10 and 100 mug ml(-1). The inhibition of PGE(2) synthesis was confirmed by the in vitro test on purified COX enzymes, showing the selectivity of prodelphinidins on COX-2. However, the prodelphinidins had no effects on COX activity in the whole blood assay. Our studies suggest that the prodelphinidins fractions from R. nigrum may be useful as an additive agent in the prevention of osteoarthritis.
Permalink :	http://hdl.handle.net/2268/13573

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