ORBi: Debois Delphine - MALDI-In Source Decay Applied to Mass Spectrometry Imaging: A New Tool for Protein Identification.

Reference : MALDI-In Source Decay Applied to Mass Spectrometry Imaging: A New Tool for Protein Ident...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Multidisciplinary, general & others Physical, chemical, mathematical & earth Sciences : Chemistry Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/13543

Title :	MALDI-In Source Decay Applied to Mass Spectrometry Imaging: A New Tool for Protein Identification.
Language :	English
Author, co-author :	Debois, Delphine [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
	Bertrand, Virginie [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie >]
	Quinton, Loïc [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
	De Pauw, Marie-Claire [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques >]
	De Pauw, Edwin [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Publication date :	15-May-2010
Journal title :	Analytical Chemistry
Publisher :	American Chemical Society
Volume :	82
Issue/season :	10
Pages :	3969-4304
Audience :	International
ISSN :	0003-2700
e-ISSN :	1520-6882
City :	Washington
Country :	DC
Keywords :	[en] Mass Spectrometry ; MALDI imaging ; Top-down proteomics
Abstract :	[en] Matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) imaging is a powerful technique giving access to the distribution of a large range of biomolecules directly from a tissue section, allowing, for example, the discovery of new pathological biomarkers. Nevertheless, one main difficulty lies in the identification of the detected species, especially proteins. MALDI-in source decay (ISD) is used to fragment ions directly in the mass spectrometer ion source. This technique does not require any special sample treatment but only the use of a specific MALDI matrix such as 2,5-dihydroxybenzoic acid or 1,5-diaminonaphthalene. MALDI-ISD is generally employed on classical, purified samples, but here we demonstrate that ISD can also be performed directly on mixtures and on a tissue slice leading to fragment ions, allowing the identification of major proteins without any further treatment. On a porcine eye lens slice, de novo sequencing was even performed. Crystallins not yet referenced in databases were identified by sequence homology with other mammalian species. On a mouse brain slice, we demonstrate that results obtained with ISD are comparable and even better than those obtained with a classical in situ digestion.
Research units :	Giga-Systems Biology and Chemical Biology ; Centre Interfacultaire d'Analyse des Résidus en Traces - CART
Funders :	Fonds Européen de Développement Régional - FEDER ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/13543
DOI :	10.1021/ac902875q

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