Reference : Improvement of the cellulose hydrolysis yields and hydrolysate concentration by manag...
Scientific journals : Article
Life sciences : Biotechnology
http://hdl.handle.net/2268/135373
Improvement of the cellulose hydrolysis yields and hydrolysate concentration by management of enzymes and substrate input
English
Jacquet, Nicolas mailto [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
Vanderghem, Caroline mailto [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
Blecker, Christophe mailto [Université de Liège - ULg > Chimie et bio-industries > Science des alim. et formul. >]
Malumba Kamba, Paul mailto [Université de Liège - ULg > Chimie et bio-industries > Labo. d'Ingénierie des procédés agro-alim. >]
Delvigne, Frank mailto [Université de Liège - ULg > Chimie et bio-industries > Bio-industries >]
Paquot, Michel mailto [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
Sep-2012
Cerevisia : Belgian Journal of Brewing and Biotechnology
37
Yes
International
1373-7163
[en] Cellulose ; Enzymatic hydrolysis ; Concentration
[en] In order to improve the hydrolysis of cellulose fiber and to obtain highly concentrated hydrolysate, two
methods based on successive addition of enzyme and substrate were assessed.
The first method, which required only substrate addition, allowed to increase by 50% the hydrolysate
concentration and to decrease by 30% enzyme units needed.
The second method highlighted the ability to reach very high concentrated hydrolysate (up to 170 g/l)
by simultaneous addition of enzyme and substrate.
In parallel, relationships between some limiting factors and the yields of hydrolysis were investigated.
In conclusion, viscosity evolution of cellulose suspension during hydrolysis step was investigated with
an aim to improve the management of enzyme and substrate addition.
http://hdl.handle.net/2268/135373

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