Reference : Improvement of the cellulose hydrolysis yields and hydrolysate concentration by manageme...

	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biotechnology
	To cite this reference:	http://hdl.handle.net/2268/135373

Title :	Improvement of the cellulose hydrolysis yields and hydrolysate concentration by management of enzymes and substrate input
Language :	English
Author, co-author :	Jacquet, Nicolas [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
	Vanderghem, Caroline [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
	Blecker, Christophe [Université de Liège - ULg > Chimie et bio-industries > Science des alim. et formul. >]
	Malumba Kamba, Paul [Université de Liège - ULg > Chimie et bio-industries > Labo. d'Ingénierie des procédés agro-alim. >]
	Delvigne, Frank [Université de Liège - ULg > Chimie et bio-industries > Bio-industries >]
	Paquot, Michel [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
Publication date :	Sep-2012
Journal title :	Cerevisia : Belgian Journal of Brewing and Biotechnology
Volume :	37
Audience :	International
ISSN :	1373-7163
Keywords :	[en] Cellulose ; Enzymatic hydrolysis ; Concentration
Abstract :	[en] In order to improve the hydrolysis of cellulose fiber and to obtain highly concentrated hydrolysate, two methods based on successive addition of enzyme and substrate were assessed. The first method, which required only substrate addition, allowed to increase by 50% the hydrolysate concentration and to decrease by 30% enzyme units needed. The second method highlighted the ability to reach very high concentrated hydrolysate (up to 170 g/l) by simultaneous addition of enzyme and substrate. In parallel, relationships between some limiting factors and the yields of hydrolysis were investigated. In conclusion, viscosity evolution of cellulose suspension during hydrolysis step was investigated with an aim to improve the management of enzyme and substrate addition.
Permalink :	http://hdl.handle.net/2268/135373

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