Reference : Cyclic lipopeptide profile of the plant-beneficial endophytic bacterium Bacillus subt...
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/135285
Cyclic lipopeptide profile of the plant-beneficial endophytic bacterium Bacillus subtilis HC8
English
Malfanova, N. [Sylvius Laboratory, Institute of Biology, Leiden University, Sylviusweg 72, Leiden, 2333 BE, Netherlands, All-Russian Research Institute for Agricultural Microbiology (ARRIAM), Saint-Petersburg-Pushkin, Russian Federation]
Franzil, Laurent mailto [Université de Liège - ULg > Chimie et bio-industries > Bio-industries >]
Lugtenberg, B. [Sylvius Laboratory, Institute of Biology, Leiden University, Sylviusweg 72, Leiden, 2333 BE, Netherlands]
Chebotar, V. [All-Russian Research Institute for Agricultural Microbiology (ARRIAM), Saint-Petersburg-Pushkin, Russian Federation]
Ongena, Marc mailto [Université de Liège - ULg > Chimie et bio-industries > Bio-industries >]
2012
Archives of Microbiology
1-7
Yes (verified by ORBi)
International
0302-8933
1432-072X
[en] Bacillus ; Cyclic lipopeptides ; Endophytic ; LC-MS
[en] In a previous study (Malfanova et al. in Microbial Biotech 4:523-532, 2011), we described the isolation and partial characterization of the biocontrol endophytic bacterium B. subtilis HC8. Using thin-layer chromatography, we have detected several bioactive antifungal compounds in the methanolic extract from the acid-precipitated supernatant of HC8. In the present study, we have further analyzed this methanolic extract using liquid chromatography-mass spectrometry. Based on the comparison of retention times and molecular masses with those of known antifungal compounds, we identified three families of lipopeptide antibiotics. These include four iturins A having fatty acyl chain lengths of C14 to C17, eight fengycins A (from C14 to C18 and from C15 to C17 containing a double bond in the acyl chain), four fengycins B (C15 to C18), and five surfactins (C12 to C16). Evaluation of the antifungal activity of the isolated lipopeptides showed that fengycins are the most active ones. To our knowledge, this is the first report of an endophytic Bacillus subtilis producing all three major families of lipopeptide antibiotics containing a very heterogeneous mixture of homologues. The questions remain open which of these lipopeptides (1) are being produced during interaction with the plant and (2) are contributing to the biocontrol activity of HC8. © 2012 The Author(s).
http://hdl.handle.net/2268/135285
10.1007/s00203-012-0823-0

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