Reference : Characterization of the resistance of SJL/J mice to pneumonia virus of mice, a model ...
Scientific journals : Article
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/135282
Characterization of the resistance of SJL/J mice to pneumonia virus of mice, a model for infantile bronchiolitis due to a respiratory syncytial virus
English
Glineur, Stéphanie mailto [Université de Liège - ULg > Département de morphologie et pathologie > Pathologie spéciale et autopsies >]
bui tran anh, dao [University of Hanoi > Pathology > > >]
Sarlet, Michaël mailto [Université de Liège - ULg > Département de morphologie et pathologie > Département de morphologie et pathologie >]
Michaux, Charles mailto [Université de Liège - ULg > Département de productions animales > Biostatistique, économie, sélection animale >]
Desmecht, Daniel mailto [Université de Liège - ULg > Département de morphologie et pathologie > Pathologie spéciale et autopsies >]
Oct-2012
PLoS ONE
Public Library of Science
7
10
e44581
Yes (verified by ORBi)
International
1932-6203
San Franscisco
CA
[en] Respiratory syncytial virus ; resistance ; segregation analysis
[en] Respiratory syncytial virus (RSV), a prominent cause of airway morbidity in children, maintains an excessive hospitalization rate despite decades of research. Host factors are assumed to influence the disease severity. As a first step toward identifying the underlying resistance mechanisms, we recently showed that inbred mouse strains differ dramatically as regards their susceptibility to pneumonia virus of mice (PVM), the murine counterpart of RSV. PVM infection in mice has been shown to faithfully mimic the severe RSV disease in human infants. This study aimed at dissecting the remarkable PVM-resistance shown by the SJL/J strain. To characterize its genetic component, we assessed clinical, physiopathological, and virological resistance/susceptibility traits in large first (F1) and second (F2) generations obtained by crossing the SJL/J (resistant) and 129/Sv (susceptible) strains. Then, to acquire conclusive in vivo evidence in support of the hypothesis that certain radiosensitive hematopoietic cells might play a significant role in PVM-resistance, we monitored the same resistance/susceptibility traits in mock- and γ-irradiated SJL/J mice. Segregation analysis showed that (i) PVM-resistance is polygenic, (ii) the resistance alleles are recessive, and (iii) all resistance-encoding alleles are concentrated in SJL/J. Furthermore, there was no alteration of SJL/J PVM resistance after immunosuppression by γ-irradiation, which suggests that adaptive immunity is not involved. We conclude that host resistance to pneumoviruses should be amenable to genetic dissection in this mouse model and that radioresistant lung epithelial cells and/or alveolar macrophages may control the clinical severity of pneumovirus-associated lung disease.
Researchers ; Professionals
http://hdl.handle.net/2268/135282

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