Reference : Direct identification of bacteria from BacT/ALERT anaerobic positive blood cultures by M...
Scientific journals : Article
Human health sciences : Laboratory medicine & medical technology
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/135093
Direct identification of bacteria from BacT/ALERT anaerobic positive blood cultures by MALDI-TOF MS: MALDI Sepsityper kit versus an in-house saponin method for bacterial extraction.
English
MEEX, Cécile mailto [Centre Hospitalier Universitaire de Liège - CHU > > Microbiologie médicale >]
Neuville, Florence []
DESCY, Julie mailto [Centre Hospitalier Universitaire de Liège - CHU > > Microbiologie médicale >]
HUYNEN, Pascale mailto [Centre Hospitalier Universitaire de Liège - CHU > > Microbiologie médicale >]
HAYETTE, Marie-Pierre mailto [Centre Hospitalier Universitaire de Liège - CHU > > Microbiologie médicale >]
De Mol, Patrick mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
MELIN, Pierrette mailto [Centre Hospitalier Universitaire de Liège - CHU > > Microbiologie médicale >]
Nov-2012
Journal of Medical Microbiology
Lippincott Williams & Wilkins
61
1511-1516
Yes (verified by ORBi)
International
0022-2615
1473-5644
London
United Kingdom
[en] MALDI-TOF ; Blood culture ; BactAlert
[en] In cases of bacteraemia, a rapid species identification of the causal agent directly from positive blood culture broths could assist clinicians in the timely targeting of empirical antimicrobial therapy. For this purpose, we evaluated the direct identification of micro-organisms from BacT/ALERT (bioMérieux) anaerobic positive blood cultures without charcoal using the Microflex matrix-assisted laser desorption/ionization (MALDI) time of flight MS (Bruker), after bacterial extraction by using two different methods: the MALDI Sepsityper kit (Bruker) and an in-house saponin lysis method. Bruker's recommended criteria for identification were expanded in this study, with acceptance of the species identification when the first three results with the best matches with the MALDI Biotyper database were identical, whatever the scores were. In total, 107 monobacterial cultures and six polymicrobial cultures from 77 different patients were included in this study. Among monomicrobial cultures, we identified up to the species level 67 and 66 % of bacteria with the MALDI Sepsityper kit and the saponin method, respectively. There was no significant difference between the two extraction methods. The direct species identification was particularly inconclusive for Gram-positive bacteria, as only 58 and 52 % of them were identified to the species level with the MALDI Sepsityper kit and the saponin method, respectively. Results for Gram-negative bacilli were better, with 82.5 and 90 % of correct identification to the species level with the MALDI Sepsityper kit and the saponin method, respectively. No misidentifications were given by the direct procedures when compared with identifications provided by the conventional method. Concerning the six polymicrobial blood cultures, whatever the extraction method used, a correct direct identification was only provided for one of the isolated bacteria on solid medium in all cases. The analysis of the time-to-result demonstrated a reduction in the turnaround time for identification ranging from 1 h 06 min to 24 h 44 min, when performing the blood culture direct identification in comparison with the conventional method, whatever the extraction method.
Professionals
http://hdl.handle.net/2268/135093
10.1099/jmm.0.044750-0

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