Reference : Isotopically labeled proteome as an internal standard for multiple reaction monitoring-b...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Human health sciences : Oncology
http://hdl.handle.net/2268/134611
Isotopically labeled proteome as an internal standard for multiple reaction monitoring-based biomarker quantification.
English
Turtoi, Andrei mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases >]
Castronovo, Vincenzo mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire >]
2012
Expert Review of Proteomics
9
3
245-8
Yes (verified by ORBi)
International
1478-9450
1744-8387
England
[en] cancer ; proteomics ; relative protein quantification ; serum ; target analysis ; tissue
[en] Multiple reaction monitoring is a mass spectrometry technology used to selectively identify and quantify a known molecule in a complex mixture. The technology has gained favor in proteomic applications, especially for biomarker quantification in human samples. For this purpose, employment of internal standard consisting of isotopically (heavy) labeled proteins is currently considered the best way of normalizing sample preparation and correcting for different ionization efficiencies. However, synthesis of heavy-labeled proteins is considered laborious and expensive. The work outlined here presents an efficient strategy of utilizing isotope-labeled amino acids in cell culture to produce heavy-labeled proteins. These are then spiked into serum and serve as internal standards to relatively quantify a large number of target proteins. The method has been applied to quantify 72 proteins in the sera of pancreatic cancer patients with remarkable efficiency and accuracy.
GIGA-Cancer
http://hdl.handle.net/2268/134611
10.1586/epr.12.27

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