Reference : Flow cytometric detection of myeloperoxidase in horse neutrophils: a novel technique in ...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/102969
Flow cytometric detection of myeloperoxidase in horse neutrophils: a novel technique in equine diagnostic research.
English
Wauters, Jella mailto [Faculty of Veterinary Medicine, Ghent University > Pharmacology, Toxicology and Biochemistry > > >]
Franck, Thierry mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Pille, Frederik [> >]
Martens, Ann [> >]
Demeyere, Kristel [> >]
Sys, Stanislas [> >]
Serteyn, Didier mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Gasthuys, Frank [> >]
Meyer, Evelyne [Faculty of Veterinary Medicine, Ghent University > Pharmacology, Toxicology and Biochemistry > > >]
2011
Veterinary Immunology and Immunopathology
144
3-4
417-22
Yes (verified by ORBi)
International
0165-2427
1873-2534
Netherlands
[en] Animals ; Coloring Agents/diagnostic use ; Flow Cytometry/veterinary ; Fluorescent Antibody Technique, Direct/veterinary ; Fluorescent Antibody Technique, Indirect/veterinary ; Horses/immunology/metabolism ; Neutrophils/enzymology ; Peroxidase/metabolism
[en] Myeloperoxidase (MPO) is a protein of interest due to its involvement in equine pathologies. Until now, results in equine diagnostic research were achieved through extracellular MPO detection. However, studying the cellular MPO content in neutrophils has revealed important insights in human diseases. This study aimed to develop a technique for the specific detection of MPO on the single cell level defining a flow cytometric protocol for the detection of both equine surface-bound and cellular MPO. Both indirect and direct labeling techniques are described which include the comparison of two secondary antibodies and two linking-fluorochromes, respectively.
Centre de l’Oxygène, Recherche et Développement - CORD
Research funded by a Ph.D. grant of the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen)
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/102969
also: http://hdl.handle.net/2268/134596
10.1016/j.vetimm.2011.09.009
Copyright (c) 2011 Elsevier B.V. All rights reserved.

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