ORBi: Wauters Jella - Flow cytometric detection of myeloperoxidase in horse neutrophils: a novel technique in equine diagnostic research.

Reference : Flow cytometric detection of myeloperoxidase in horse neutrophils: a novel technique in ...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Veterinary medicine & animal health
	To cite this reference:	http://hdl.handle.net/2268/134596

Title :	Flow cytometric detection of myeloperoxidase in horse neutrophils: a novel technique in equine diagnostic research.
Language :	English
Author, co-author :	Wauters, Jella [Faculty of Veterinary Medicine, Ghent University > Pharmacology, Toxicology and Biochemistry > > >]
	Franck, Thierry [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
	Pille, Frederik [> >]
	Martens, Ann [> >]
	Demeyere, Kristel [> >]
	Sys, Stanislas [> >]
	Serteyn, Didier [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
	Gasthuys, Frank [> >]
	Meyer, Evelyne [Faculty of Veterinary Medicine, Ghent University > Pharmacology, Toxicology and Biochemistry > > >]
Publication date :	15-Dec-2011
Journal title :	Veterinary Immunology and Immunopathology
Volume :	144
Issue/season :	3-4
Pages :	417-22
Audience :	International
ISSN :	0165-2427
Country :	Netherlands
Keywords :	[en] Animals ; Coloring Agents/diagnostic use ; Flow Cytometry/veterinary ; Fluorescent Antibody Technique, Direct/veterinary ; Fluorescent Antibody Technique, Indirect/veterinary ; Horses/immunology/metabolism ; Neutrophils/enzymology ; Peroxidase/metabolism
Abstract :	[en] Myeloperoxidase (MPO) is a protein of interest due to its involvement in equine pathologies. Until now, results in equine diagnostic research were achieved through extracellular MPO detection. However, studying the cellular MPO content in neutrophils has revealed important insights in human diseases. This study aimed to develop a technique for the specific detection of MPO on the single cell level defining a flow cytometric protocol for the detection of both equine surface-bound and cellular MPO. Both indirect and direct labeling techniques are described which include the comparison of two secondary antibodies and two linking-fluorochromes, respectively.
Research units :	Centre de l’Oxygène, Recherche et Développement - CORD
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/134596

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