Reference : An in vitro whole blood model to test the effects of different stimuli conditions on ...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/134587
An in vitro whole blood model to test the effects of different stimuli conditions on the release of myeloperoxidase and elastase by equine neutrophils.
English
Ceusters, Justine mailto [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Serteyn, Didier mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
MINGUET, Grégory mailto [Centre Hospitalier Universitaire de Liège - CHU > > Anesthésie et réanimation >]
de la Rebière de Pouyade, Geoffroy mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Romainville, Jennifer [Université de Liège - ULg > Centre de l'oxygène : Recherche et développement (C.O.R.D.) > > >]
Deby-Dupont, Ginette P. [Université de Liège - ULg > Centre de l'oxygène : Recherche et développement (C.O.R.D.) > > > >]
Mouithys-Mickalad, Ange mailto [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Franck, Thierry mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
15-Dec-2012
Veterinary Immunology and Immunopathology
150
3-4
221-7
Yes (verified by ORBi)
International
0165-2427
Netherlands
[en] myeloperoxidase ; neutrophils ; degranulation ; whole blood ; ex-vivo
[en] Horses are particularly sensitive and exposed to excessive inflammatory responses evolving toward an important stimulation of polymorphonuclear neutrophils (PMNs). The aim of this work was to stimulate equine neutrophils in whole blood and to evaluate their response by measuring the release of total and active myeloperoxidase (MPO) and total elastase, considered as markers of neutrophil stimulation and degranulation. Because of the critical importance of the concomitant presence of LPS and TNF-alpha in equine pathological situations, we combined these two natural mediators to stimulate PMN and compared the response with those obtained after the PMN stimulation with each mediator used alone and well-known artificial stimulation systems such as 12-phorbol 13-myristate acetate (PMA) and the combination of cytochalasin B (CB) and N-formyl-methionyl-leucyl-phenylalanine (fMLP). All the activation systems, PMA, CB/fMLP, TNF-alpha, LPS and LPS/TNF-alpha, induced a significant release of total MPO in whole blood but only the combinations CB/fMLP and LPS/TNF-alpha significantly favored the release of active MPO. Regarding the total elastase, we did not observe a significant release in all the stimulated conditions except with PMA. It appears clearly that the choice of the neutrophil stimulation model is fundamental for the selection of potentially active pharmacological agents, especially on MPO activity.
Centre de l’Oxygène, Recherche et Développement - CORD
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/134587
Copyright (c) 2012 Elsevier B.V. All rights reserved.

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Ceusters-PMN2012.pdfPublisher postprint451.62 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.