Article (Scientific journals)
An in vitro whole blood model to test the effects of different stimuli conditions on the release of myeloperoxidase and elastase by equine neutrophils.
Ceusters, Justine; Serteyn, Didier; MINGUET, Grégory et al.
2012In Veterinary Immunology and Immunopathology, 150 (3-4), p. 221-7
Peer Reviewed verified by ORBi
 

Files


Full Text
Ceusters-PMN2012.pdf
Publisher postprint (462.45 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
myeloperoxidase; neutrophils; degranulation; whole blood; ex-vivo
Abstract :
[en] Horses are particularly sensitive and exposed to excessive inflammatory responses evolving toward an important stimulation of polymorphonuclear neutrophils (PMNs). The aim of this work was to stimulate equine neutrophils in whole blood and to evaluate their response by measuring the release of total and active myeloperoxidase (MPO) and total elastase, considered as markers of neutrophil stimulation and degranulation. Because of the critical importance of the concomitant presence of LPS and TNF-alpha in equine pathological situations, we combined these two natural mediators to stimulate PMN and compared the response with those obtained after the PMN stimulation with each mediator used alone and well-known artificial stimulation systems such as 12-phorbol 13-myristate acetate (PMA) and the combination of cytochalasin B (CB) and N-formyl-methionyl-leucyl-phenylalanine (fMLP). All the activation systems, PMA, CB/fMLP, TNF-alpha, LPS and LPS/TNF-alpha, induced a significant release of total MPO in whole blood but only the combinations CB/fMLP and LPS/TNF-alpha significantly favored the release of active MPO. Regarding the total elastase, we did not observe a significant release in all the stimulated conditions except with PMA. It appears clearly that the choice of the neutrophil stimulation model is fundamental for the selection of potentially active pharmacological agents, especially on MPO activity.
Research center :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Ceusters, Justine ;  Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Serteyn, Didier  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
MINGUET, Grégory ;  Centre Hospitalier Universitaire de Liège - CHU > Anesthésie et réanimation
de la Rebière de Pouyade, Geoffroy  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Romainville, Jennifer;  Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Dupont, Ginette ;  Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Mouithys-Mickalad, Ange ;  Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Franck, Thierry  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
An in vitro whole blood model to test the effects of different stimuli conditions on the release of myeloperoxidase and elastase by equine neutrophils.
Publication date :
15 December 2012
Journal title :
Veterinary Immunology and Immunopathology
ISSN :
0165-2427
eISSN :
1873-2534
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
150
Issue :
3-4
Pages :
221-7
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2012 Elsevier B.V. All rights reserved.
Available on ORBi :
since 22 November 2012

Statistics


Number of views
93 (30 by ULiège)
Number of downloads
6 (4 by ULiège)

Scopus citations®
 
6
Scopus citations®
without self-citations
4
OpenCitations
 
6

Bibliography


Similar publications



Contact ORBi