An in vitro whole blood model to test the effects of different stimuli conditions on the release of myeloperoxidase and elastase by equine neutrophils.
[en] Horses are particularly sensitive and exposed to excessive inflammatory responses evolving toward an important stimulation of polymorphonuclear neutrophils (PMNs). The aim of this work was to stimulate equine neutrophils in whole blood and to evaluate their response by measuring the release of total and active myeloperoxidase (MPO) and total elastase, considered as markers of neutrophil stimulation and degranulation. Because of the critical importance of the concomitant presence of LPS and TNF-alpha in equine pathological situations, we combined these two natural mediators to stimulate PMN and compared the response with those obtained after the PMN stimulation with each mediator used alone and well-known artificial stimulation systems such as 12-phorbol 13-myristate acetate (PMA) and the combination of cytochalasin B (CB) and N-formyl-methionyl-leucyl-phenylalanine (fMLP). All the activation systems, PMA, CB/fMLP, TNF-alpha, LPS and LPS/TNF-alpha, induced a significant release of total MPO in whole blood but only the combinations CB/fMLP and LPS/TNF-alpha significantly favored the release of active MPO. Regarding the total elastase, we did not observe a significant release in all the stimulated conditions except with PMA. It appears clearly that the choice of the neutrophil stimulation model is fundamental for the selection of potentially active pharmacological agents, especially on MPO activity.
Research center :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Ceusters, Justine ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
MINGUET, Grégory ; Centre Hospitalier Universitaire de Liège - CHU > Anesthésie et réanimation
de la Rebière de Pouyade, Geoffroy ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Romainville, Jennifer; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Dupont, Ginette ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Mouithys-Mickalad, Ange ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Franck, Thierry ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
An in vitro whole blood model to test the effects of different stimuli conditions on the release of myeloperoxidase and elastase by equine neutrophils.
Publication date :
15 December 2012
Journal title :
Veterinary Immunology and Immunopathology
ISSN :
0165-2427
eISSN :
1873-2534
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
150
Issue :
3-4
Pages :
221-7
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2012 Elsevier B.V. All rights reserved.
Benbarek H., Deby-Dupont G., Caudron I., Deby C., Lamy M., Serteyn D. Failure of lipopolysaccharides to directly trigger the chemiluminescence response of isolated equine polymorphonuclear leukocytes. Vet. Res. Commun. 1997, 21:477-482.
Benbarek H., Deby-Dupont G., Caudron I., Grülke S., Deby C., Lamy M., Serteyn D. Interactions between lipopolysaccharides and blood factors on the stimulation of equine polymorphonuclear neutrophils. Vet. Immunol. Immunopathol. 1998, 64:313-322.
Benbarek H., Deby-Dupont G., Deby C., Serteyn D. Direct stimulation of the oxidative activity of isolated equine neutrophils by TNF-alpha and IL-1beta. Vet. Immunol. Immunopathol. 2008, 15:101-106.
Berczi I., Bertok L., Bereznai T. Comparative studies on the toxicity of Escherichia coli-lipopolysaccharide endotoxin in various animal species. Can. J. Microbiol. 1966, 12:1070-1071.
Bochsler P.N., Slauson D.O., Neilsen N.R. Secretory activity of equine polymorphonuclear leukocytes: stimulus specificity and priming effects of bacterial lipopolysaccharide. Vet. Immunol. Immunopathol. 1992, 31:241-253.
Borregaard N., Cowland J.B. Granules of the human neutrophilic polymorphonuclear leukocytes. Blood 1997, 89:3503-3521.
Braga P.C., Dal Sasso M., Culici M., Bianchi T., Bordoni L., Marabini L. Anti-inflammatory activity of thymol: inhibitory effect on the release of human neutrophil elastase. Pharmacology 2006, 77:130-136.
Brazil T.J., Rossi A.G., Haslett C., McGorum B., Dixon P.M., Chilvers E.R. Priming induces functional coupling of N-formyl-methionyl-leucyl-phenylalanine receptors in equine neutrophils. J. Leukoc. Biol. 1998, 63:380-388.
Burrows G.E. Dose-response of ponies to parenteral Escherichia coli endotoxin. Can. J. Comp. Med. 1981, 45:207-210.
Dagleish M.P., Brazil T.J., Scudamore C.L. Potentiation of the extracellular release of equine neutrophil elastase and alhpa-1-proteinase inhibitor by a combination of two bacterial cell wall components: fMLP and LPS. Equine Vet. J. 2003, 35:35-39.
De la Rebière de Pouyade G., Serteyn D., Deby-Dupont G., Franck T. Method for co-purification of equine neutrophil elastase and myeloperoxidase from a limited blood volume. Res. Vet. Sci. 2009, 87:358-363.
De la Rebière de Pouyade G., Riggs L.M., Moore J.N., Franck T., Deby-Dupont G., Hurley D.J., Serteyn D. Equine neutrophil elastase in plasma laminar tissue, and skin of horses administered black walnut heartwood extract. Vet. Immunol. Immunopathol. 2010, 135:181-187.
De la Rebière de Pouyade G., Franck T., Salciccia A., Deby-Dupont G., Grulke S., Vander Heyden L., Sandersen C., Serteyn D. Development of an enzyme-linked immunosorbent assay for equine neutrophil elastase measurement in blood: preliminary application to colic cases. Vet. Immunol. Immunopathol. 2010, 135:282-288.
Deby-Dupont G., Deby C., Lamy M. Neutrophil myeloperoxidase revisited: it's role in health and disease. Intensivmed. Notfallmed. 1999, 36:500-513.
Dorn C.R., Coffman J.R., Schmidt D.A., Garner H.E., Addison J.B., McCune E.L. Neutropenia and salmonellosis in hospitalized horses. J. Am. Vet. Med. Assoc. 1975, 166:65-67.
Dubin A., Koj A. Proteinase inhibitors from horse plasma and leucocytes: properties and biologic functions. Biomed. Biochim. Acta 1986, 45:1391-1396.
Faymonville M.E., Pincemail J., Duchateau J., Paulus J.M., Adam A., Deby-Dupont G., Deby C., Albert A., Larbuisson R., Limet R. Myeloperoxidase and elastase as markers of leukocyte activation during cardiopulmonary bypass in humans. J. Thorac. Cardiovasc. Surg. 1991, 102:309-317.
Franck T., Grulke S., Deby-Dupont G., Deby C., Duvivier H., Peters F., Serteyn D. Development of an enzyme-linked immunosorbent assay for specific equine neutrophil myeloperoxidase measurement in blood. J. Vet. Diagn. Invest. 2005, 17:412-419.
Franck T., Kohnen S., Deby-Dupont G., Grülke S., Deby C., Serteyn D. A specific method for measurement of equine active myeloperoxidase in biological samples and in in vitro tests. J. Vet. Diagn. Invest. 2006, 18:326-334.
Franck T., Kohnen S., de la Rebière G., Deby-Dupont G., Deby C., Niesten A., Serteyn D. Activation of equine neutrophils by phobol myristate acetate or N-formyl-methionyl-leucyl-phenylalanine induces a different response in reactive oxygen species production and release of active myeloperoxidase. Vet. Immunol. Immunopathol. 2009, 130:243-250.
Franck T., Kohnen S., Zouaoui Boudjeltia K., Van Antwerpen P., Bosseloir A., Niesten A., Gach O., Nys M., Deby-Dupont G., Serteyn D. A new easy method for specific measurement of active myeloperoxidase in human biological fluids and tissue extracts. Talenta 2009, 80:723-729.
Gougerot-Pocidalo M.A., el Benna J., Elbim C., Chollet-Martin S., Dang M.C. Regulation of human neutrophil oxidative burst by pro- and anti-inflammatory cytokines. J. Soc. Biol. 2002, 196:37-46.
Grulke S., Franck T., Gangl M., Péters F., Salciccia A., Deby-Dupont G., Serteyn D. Myeloperoxidase assay in plasma and peritoneal fluids of horses with gastro-intestinal diseases. Can. J. Vet. Res. 2008, 72:37-42.
Hunt R.J., Allen D., Moore J.N. Effect of the endotoxin on equine digital hemodynamics and starling forces. Am. J. Vet. Res. 1987, 19:25-28.
King C.C., Jefferson M.M., Thomas E.L. Secretion and inactivation of myeloperoxidase by isolated neutrophils. J. Leukoc. Biol. 1997, 61:293-302.
Klebanoff S.J., Vadas M.A., Harlan J.M., Sparks L.H., Gamble J.R., Agosti J.M., Waltersdorph A.M. Stimulation of neutrophils by tumor necrosis factor. J. Immunol. 1986, 136:4220-4225.
Klebanoff S.J. Myeloperoxidase: friend and foe. J. Leukoc. Biol. 2005, 77:598-625.
Kyaw W.O., Uhlig A., Köller G., Sack U., Schusser G.F. Free hemoglobin and tumor necrosis factor-alpha in the blood of horses with colic or acute colitis. Berl. Munch. Tierarztl. Wochenschr. 2008, 121:440-445.
Langhorst J., Elsenbruch S., Koelzer J., Rueffer A., Michalsen A., Dobos G.J. Non-invasive markers in the assessment of intestinal inflammation in inflammatory bowel diseases: performance of fecal lactoferrin, calprotectin, and PMN-elastase, CRP, and clinical indices. Am. J. Gastroenterol. 2008, 103:162-169.
Lee W.L., Harrison R.E., Grinstein S. Phagocytosis by neutrophils. Microbes Infect. 2003, 5:1299-1306.
Meyers K., Reed S., Keck M., Clem M., Baily W. Circulating endotoxin-like substance(s) and altered hemostasis in horse with gastrointestinal disorders: an interim report. Am. J. Vet. Res. 1982, 43:2233-2238.
Minguet, G.,de la Rebière, G., Franck, T., Joris, J., Serteyn, D., Sandersen, C. Sevoflurane inhibits equine myeloperoxidase release and activity in vitro. Vet. Anaesth. Analg., in press. doi:10.1111/j.1467-2995.2012.00786.x.
Partrick D.A., Moore F.A., Moore E.E., Barnett C.C., Silliman C.C. Neutrophil priming and activation in the pathogenesis of post-injury multiple organ failure. New Horiz. 1996, 4:194-210.
Sample A.K., Czuprynski C.J. Bovine neutrophils chemiluminescence is preferentially stimulated by homologous IL-1, but inhibited by the human IL-1 receptor antagonist. Vet. Immunol. Immunopathol. 1994, 41:165-172.
Seethanathan P., Bottoms G.D., Schafer K. Characterization of release of tumor necrosis factor, interleukin-1, and superoxide anion from equine white blood cells in response to endotoxin. Am. J. Vet. Res. 1990, 51:1221-1225.
Serteyn D., Grulke S., Franck T., Mouithys-Mickalad A., Deby-Dupont G. La myéloperoxydase des neutrophiles, une enzyme de défense aux capacités oxydantes. Ann. Med. Vet. 2003, 147:79-93.
Serteyn D., Sandersen C., Lejeune J.P., de la Rebière de Pouyade G., Ceusters J., Mouithys-Mickalad A., Niesten A., Fraipont A., Van Erck E., Goachet A.G., Robert C., Leclerc J.L. Effect of a 120km endurance race on plasma and muscular neutrophil elastase and myeloperoxidase concentrations in horses. Equine Vet. J. 2010, 42(Suppl. 38):275-279.
Swain S.D., Rohn T.T., Quinn M.T. Neutrophil priming in host defense: role of oxidants as priming agents. Antioxid. Redox Signal. 2002, 4:69-83.
Weiss D.J., Evanson O.A. Evaluation of lipopolysaccharide-induced activation of equine neutrophils. Am. J. Vet. Res. 2002, 63:811-815.
Zhang R., Brennan M.L., Fu X., Aviles R.J., Pearce G.L., Penn M.S., Topol E.J., Sprecher D.L., Hazen S.L. Association between myeloperoxidase levels and risk of coronary artery disease. JAMA 2001, 286:2136-2142.