Les souches pathogènes d'Escherichia coli chez les chiens et les chats: III) Données bactériologiques et cliniques sur les souches nécrotoxinogènes et sur celles positives pour des adhésines
[en] The purpose of this study was to characterize 25 necrotoxigenic Escherichia coli type 1 (NTEC1), one necrotoxigenic E coli type 2 (NTEC2) and 16 non-necrotoxigenic E coli isolated, between 1979 and 1993, from feces, intestinal content or internal organs of dogs and cats using a uniform and standardized typing scheme, as previously described on NTEC1 and NTEC2 isolates from cattle, humans and pigs (Mainil et al, 1999). The results obtained by colony hybridization and PCR on the NTEC1 isolates were homogeneous: eighteen NTEC1 isolates harboured prs- and sfa/foc-related DNA sequences; five NTEC1 isolates harboured sfa/foc-related DNA sequences; one NTEC1 isolate harboured prs-related DNA sequences; and one was negative. The only NTEC2 isolate was also negative. Only a few NTEC1 isolates were positive for f17-, afa- or cdt-related DNA sequences. All NTEC1 and the NTEC2 isolates were haemolytic on blood agar and were resistant to the bactericidal activity of the serum (one exception). By contrast a very few produced an aerobactin or a colicin. The strains belonged to various serogroups and many could not be identified with the immunsera used. On the other hand all but one NTEC1 isolates belonged to biotype 9. The most typical profile identifed for NTEC1 from dogs and cats was: CNF1+Prs+S+Hly+Bio9+. The results obtained on the non-necrotoxigenic E. coli isolates were more heterogeneous: two of them harboured pap/prs- and sfa/foc-related DNA sequences; ten harboured pap/prs-related DNA sequences; and four, other DNA sequences. The results of the phenotypic assays were more heterogeneous also. NTEC1 strains were isolated from essentially young puppies and kitties (< 3 months for 16 out of 22), with sometimes older animals (up to 12 years). The digestive pathologies were non haemorrhagic diarrhea/enteritis essentially. When haemorrhagic diarrhea/enteritis were observed, other infectious agents were present or strongly suspected (parvovirus, Salmonella). Extraintestinal pathologies were septicaemic or systemic colibacillosis, with one case of necro-haemorrhagic metritis. NTEC1 and non-NTEC were not frequently antibiotic resistant. The most frequent resistant were to ampicillin, trimethoprim/sulfonamides and neomycin. [en] The purpose of this study was to characterize 25 necrotoxigenic Escherichia coli type 1 (NTEC1), one necrotoxigenic E coli type 2 (NTEC2) and 16 non-necrotoxigenic E coli isolated, between 1979 and 1993, from feces, intestinal content or internal organs of dogs and cats using a uniform and standardized typing scheme, as previously described on NTEC1 and NTEC2 isolates from cattle, humans and pigs (Mainil et al, 1999). The results obtained by colony hybridization and PCR on the NTEC1 isolates were homogeneous: eighteen NTEC1 isolates harboured prs- and sfa/foc-related DNA sequences; five NTEC1 isolates harboured sfa/foc-related DNA sequences; one NTEC1 isolate harboured prs-related DNA sequences; and one was negative. The only NTEC2 isolate was also negative. Only a few NTEC1 isolates were positive for f17-, afa- or cdt-related DNA sequences. All NTEC1 and the NTEC2 isolates were haemolytic on blood agar and were resistant to the bactericidal activity of the serum (one exception). By contrast a very few produced an aerobactin or a colicin. The strains belonged to various serogroups and many could not be identified with the immunsera used. On the other hand all but one NTEC1 isolates belonged to biotype 9. The most typical profile identifed for NTEC1 from dogs and cats was: CNF1+Prs+S+Hly+Bio9+. The results obtained on the non-necrotoxigenic E. coli isolates were more heterogeneous: two of them harboured pap/prs- and sfa/foc-related DNA sequences; ten harboured pap/prs-related DNA sequences; and four, other DNA sequences. The results of the phenotypic assays were more heterogeneous also. NTEC1 strains were isolated from essentially young puppies and kitties (< 3 months for 16 out of 22), with sometimes older animals (up to 12 years). The digestive pathologies were non haemorrhagic diarrhea/enteritis essentially. When haemorrhagic diarrhea/enteritis were observed, other infectious agents were present or strongly suspected (parvovirus, Salmonella). Extraintestinal pathologies were septicaemic or systemic colibacillosis, with one case of necro-haemorrhagic metritis. NTEC1 and non-NTEC were not frequently antibiotic resistant. The most frequent resistant were to ampicillin, trimethoprim/sulfonamides and neomycin.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Wilbaux, Myriam
Jacquemin, Etienne
Oswald, Eric
Imberechts, Hein
Van Bost, Sigrid
Language :
French
Title :
Les souches pathogènes d'Escherichia coli chez les chiens et les chats: III) Données bactériologiques et cliniques sur les souches nécrotoxinogènes et sur celles positives pour des adhésines
BERTIN A., MARTIN C., GIRARDEAU J.P., POHL P., CONTREPOIS M. Association of genes encoding P fimbriae, CS31A antigen and EAST I toxin among CNF1-producing Escherichia coli strains from cattle with septicemia and diarrhea. FEMS Microbiol. Lett., 1998, 162, 235-239.
BERTIN A., MARTIN C., OSWALD E., GIRARDEAU J.P. Rapid and specific detection of F17-related pilin and adhesin genes in diarrheic and septicemic Escherichia coli strains by multiplex PCR. J. Clin. Microbiol., 1996, 34, 2921-2928.
BETTELHEIM K.A. Escherichia coli in the normal flora of humans and animals. In: Sussman M. (Ed.), Escherichia coli : Mechanisms of virulence. Cambridge University Press : Cambridge, 1997, 85-109.
BEUTIN L. Escherichia coli as a pathogen in dogs and cats. Vet. Res., 1999, 30, 285-298.
BLANCO J., ALONSO M.P., GONZALEZ E.A., BLANCO M., GARABAL J.I. Virulence factors of bacteraemic Escherichia coli with particular reference to production of cytotoxic necrotizing factor (CNF) by P-fimbriated strains. J. Med. Microbiol., 1990, 31, 175-183.
BLANCO J., BLANCO M., ALONSO M.P., BLANCO J.E., GONZALEZ E.A., GARABAL J.I. Characteristics of haemolytic Escherichia coli with particular reference to production of cytotoxic necrotizing factor type 1 (CNF1). Res. Microbiol., 1992, 143, 869-878.
BLANCO M., BLANCO J.E., ALONSO M.P., MORA A., BALSALOBRE C., MUNOA F., JUAREZ A., BLANCO J. Detection of pap, sfa and afa adhesin-encoding operons in uropathogenic Escherichia coli strains: relationship with expression of adhesins and production of toxins. Res. Microbiol., 1997, 148, 745-755.
BROES A. Les Escherichia coli pathogènes du chien et du chat. Ann. Méd. Vét., 1993, 137, 377-384.
BROES A., FAIRBROTHER J.M., MAINIL J.G., HAREL J., LARIVIERE S. Phenotypic and genotypic characterization of enterotoxigenic Escherichia coli serotype O8:KX105 and O8:K2829 strains isolated from piglets with diarrhea. J. Clin. Microbiol., 1988, 26, 2402-2409.
COUTURIER M., BEX F., BERGQUIST P.L., MAAS W.K. Identification and classification of bacterial plasmids. Microbiol. Rev., 1988, 52, 305-317.
COWAN and STEEL's manual for identification of medical bacteria (Barrow G.I., Feltham R.K.A. eds). 3rd ed. (1993). New-York : Cambridge University Press, p194.
DE RYCKE J., MILON A., OSWALD E. Necrotoxic Escherichia coli (NTEC) : two emerging categories of human and animal pathogens. Vet. Res., 1999, 30, 221-234.
DOZOIS C.M., CLÉMENT S., DESAUTELS C., OSWALD E., FAIRBROTHER J.M. Expression of P, S, and F1C adhesins by cytotoxic necrotizing factor 1-producing Escherichia coli from septicemic and diarrheic pigs. FEMS Microbiol. Lett., 1997, 152, 307-312.
DOZOIS C., CURTISS R. III. Pathogenic diversity of Escherichia coli and the emergence of "exotic" islands in the gene stream. Vet. Res., 1999, 30, 157-179.
FOURNUT S., DOZOIS C.M., ODIN M. DESAUTELS C., PERES S., HERAULT F., DAIGLE F., SEGAFREDO C., LAFFITE J., OSWALD E., FAIRBROTHER J.M., OSWALD I.P. Lack of a role of cytotoxic necrotizing factor 1 toxin from Escherichia coli in bacterial pathogenicity and host cytokine response in infected germfree piglets. Infect. Immun., 2000, 68, 839-847.
GERARDIN J., LALIOUI L., JACQUEMIN E., LE BOUGUENEC C., MAINIL J.G. The afa-related gene cluster in necrotoxigenic and other Escherichia coli from animals belongs to the afa-8 variant. Vet. Microbiol., 2000, 76, 175-184.
HACKER J., KAPER J.B. Pathogenicity islands and the evolution of microbes. Annu. Rev. Microbiol., 2000, 54, 641-679.
JOHNSON J.R., O'BRYAN T.T., LOW D.A., LING G., DELAVARI P., FASCHING C., RUSSO T.A., CARLINO U., STELL A.L. Evidence for commonality between canine and human extraintestinal pathogenic Escherichia coli strains that express papG allele III. Infect. Immun., 2000, 68, 3327-3336.
JOHNSON J.R., STELL J.J. A novel multiply-primed polymerase chain reaction assay for identificationof variant papG genes encoding the GaI(α1-4)GaI-binding PapG adhesins of Escherichia coli. J. Infect. Dis., 1996, 173, 920-926.
JOHNSON J.R., STELL A.L. Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise. J. Infect. Dis., 2000, 181, 261-272.
LALIOUI L., JOUVE M., GOUNON P., LE BOUGUÉNEC C. Molecular cloning and characterization of the afa-7 and afa-8 gene clusters encoding afimbrial adhesins in Escherichia coli strains associated with diarrhea or septicaemia in calves. Infect. Immun., 1999, 67, 5048-5059.
LE BOUGUÉNEC C., ARCHAMBAUD M., LABIGNE A. Rapid and specific detection of the pap, afa, and sfa adhesin-encoding operons in uropathogenic Escherichia coli strains by polymerase chain reaction. J. Clin. Microbiol., 1992, 30, 1189-1193.
MAINIL J. Shiga/Verocytotoxins and Shiga/Verotoxigenic Escherichia coli in animals. Vet. Res., 1999, 30, 235-258.
MAINIL J. Le point des connaissances sur les entérites à Escherichia coli chez le veau. Ann. Méd. Vét., 2000, 144, 121-136.
MAINIL J., BEZ S., JACQUEMIN E., KAECKENBEECK A. Les souches pathogènes d'Escherichia coli chez les chiens et chats : I) Détection des souches entérotoxinogènes (ETEC), entéropathogènes (EPEC), vérotoxinogènes (VTEC), entérohémorragiques (EHEC) et nécrotoxinogènes (NTEC). Ann. Méd. Vét., 1998, 142, 39-46.
MAINIL J.G., GERARDIN J., JACQUEMIN E. Identification of the F17 fimbrial subunit-and adhesin-encoding (f17A and f17G) gene variants in necrotoxigenic Escherichia coli from cattle, pigs and humans. Vet. Microbiol., 2000a, 73, 327-335.
MAINIL J.G., JACQUEMIN E., HÉRAULT F., OSWALD E. Presence of pap-, sfa-, and afa-related sequences in necrotoxigenic Escherichia coli isolates from cattle: evidence for new variants of the AFA family. Can. J. Vet. Res., 1997, 61, 193-199.
MAINIL J.G., JACQUEMIN E., POHL P., FAIRBROTHER J.M., ANSUINI A., LE BOUGUÉNEC C., BALL H.J., DE RYCKE J., OSWALD E. Comparison of necrotoxigenic Escherichia coli from farm animals and from humans. Vet. Microbiol., 1999, 70, 123-135.
MAINIL J., JANSSEN L., CHARLIER G., JACQUEMIN E., CHINA B., GOFFAUX F. Les souches pathogènes d'Escherichia coli chez les chiens et chats: II) Données cliniques et bactériologiques sur les souches entéropathogènes. Ann. Méd. Vét., 2000b, 144, 335-343.
MILON A., OSWALD E., DE RYCKE J. Rabbit EPEC : a model for the study of enteropathogenic Escherichia coli. Vet. Res., 1999, 30, 203-220.
NAGY B., FEKETE P.Zs. Enterotoxigenic Escherichia coli in farm animals. Vet. Res., 1999, 30, 259-284.
OELSCHLAEGER T.A., HACKER J. Pathogenicity islands and their role in virulence of bacteria. In : Duffy G., Garvey P., Coia J., Wasteson Y., McDowell D.A. (Eds), Verocytotoxigenic Escherichia coli in Europe -Pathogenicity and virulence. Proceedings of the IIIrd Workshop of the European Concerted Action CT98-3935, Liège, Belgique, 2000, 73-85.
OSWALD E., DE RYCKE J., LINTERMANS P., VAN MUYLEM K., MAINIL J., DAUBE G., POHL P. Virulence factors associated with the cytotoxic necrotizing factor type 2 in bovine diarrheic and septicaemic strains of Escherichia coli. J. Clin. Microbiol., 1991, 29, 2522-2527.
OSWALD E., POHL P., JACQUEMIN E., LINTERMANS P., VAN MUYLEM K., O'BRIEN A.D., MAINIL J. Specific DNA probes to detect Escherichia coli strains producing cytotoxic necrotising factor type 1 or type 2. J. Med. Microbiol., 1994, 40, 428-434.
PEETERS J. Escherichia coli infections in rabbits, cats, dogs, goats and horses. In: Gyles C.L. (Ed.), Escherichia coli in domestic animals and humans. CAB International : Wallingford, 1994, 261-283.
POHL P., DAUBE G., MAINIL J., LINTERMANS P., KAECKENBEECK A., OSWALD E. Virulence factors and phenotypes of sixty-one strains of Escherichia coli of bovine origin, producing the cytotoxic necrotizing factor type 1. Ann. Rech. Vét., 1992, 23, 83-91.
POHL P., OSWALD E., VAN ROBAEYS G., STOCKMANS F., LINTERMANS P., MAINIL J. Tests simples pour le diagnostic présumé des Escherichia coli isolées principalement chez les bovins et productrices des cytotoxines nécrosantes (CNF1). Ann. Méd. Vét., 1993, 137, 503-505.
SAUNDERS J.R. Bacterial plasmids and their association with virulence. In : Duffy G., Garvey P., Coia J., Wasteson Y., McDowell D.A. (Eds), Verocytotoxigenic Escherichia coli in Europe - Pathogenicity and virulence. Proceedings of the IIIrd Workshop of the European Concerted Action CT98-3935, Liège, Belgique, 2000, 136-139.
SMITH H.W. Observations on Escherichia coli infection in calves. In: Rutter, J.M. (Ed.), Proceedings of the First Seminar on Pathology in the CEC Programme of Coordination of Research in Beef Production. Perinatal III-Health in Calves, 1975, 281-329.
TOTH I. Phages and their role in virulence of bacteria. In : Duffy G., Garvey P., Coia J., Wasteson Y., McDowell D.A. (Eds), Verocytotoxigenic Escherichia coli in Europe - Pathogenicity and virulence. Proceedings of the IIIrd Workshop of the European Concerted Action CT98-3935, Liège, Belgique, 2000, 1-10.
VAN BOST S., BABE M.H., JACQUEMIN E., MAINIL J. Characteristics of necrotoxigenic Escherichia coli isolated from septicemic and diarrheic calves between 1958 and 1970. Vet. Microbiol., 2001a, 82, 31 1-320.
VAN BOST S., ROELS S., MAINIL J. Necrotoxigenic Escherichia coli type 2 invade and cause diarrhea during experimental infection in colostrum restricted newborn calves. Vet. Microbiol., 2001b, 81, 315-329.
WRAY C., PIERCY D.W.T., CARROLL P.J., COOLEY W.A. Experimental infection of neonatal pigs with CNF toxin-producing strains of Escherichia coli. Res. Vet. Sci., 1993, 54, 290-298.