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Les souches pathogènes d'Escherichia coli chez les chiens et les chats: III) Données bactériologiques et cliniques sur les souches nécrotoxinogènes et sur celles positives pour des adhésines
Mainil, Jacques; Wilbaux, Myriam; Jacquemin, Etienne et al.
2001In Annales de Médecine Vétérinaire, 145 (6), p. 343-354
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Abstract :
[en] The purpose of this study was to characterize 25 necrotoxigenic Escherichia coli type 1 (NTEC1), one necrotoxigenic E coli type 2 (NTEC2) and 16 non-necrotoxigenic E coli isolated, between 1979 and 1993, from feces, intestinal content or internal organs of dogs and cats using a uniform and standardized typing scheme, as previously described on NTEC1 and NTEC2 isolates from cattle, humans and pigs (Mainil et al, 1999). The results obtained by colony hybridization and PCR on the NTEC1 isolates were homogeneous: eighteen NTEC1 isolates harboured prs- and sfa/foc-related DNA sequences; five NTEC1 isolates harboured sfa/foc-related DNA sequences; one NTEC1 isolate harboured prs-related DNA sequences; and one was negative. The only NTEC2 isolate was also negative. Only a few NTEC1 isolates were positive for f17-, afa- or cdt-related DNA sequences. All NTEC1 and the NTEC2 isolates were haemolytic on blood agar and were resistant to the bactericidal activity of the serum (one exception). By contrast a very few produced an aerobactin or a colicin. The strains belonged to various serogroups and many could not be identified with the immunsera used. On the other hand all but one NTEC1 isolates belonged to biotype 9. The most typical profile identifed for NTEC1 from dogs and cats was: CNF1+Prs+S+Hly+Bio9+. The results obtained on the non-necrotoxigenic E. coli isolates were more heterogeneous: two of them harboured pap/prs- and sfa/foc-related DNA sequences; ten harboured pap/prs-related DNA sequences; and four, other DNA sequences. The results of the phenotypic assays were more heterogeneous also. NTEC1 strains were isolated from essentially young puppies and kitties (< 3 months for 16 out of 22), with sometimes older animals (up to 12 years). The digestive pathologies were non haemorrhagic diarrhea/enteritis essentially. When haemorrhagic diarrhea/enteritis were observed, other infectious agents were present or strongly suspected (parvovirus, Salmonella). Extraintestinal pathologies were septicaemic or systemic colibacillosis, with one case of necro-haemorrhagic metritis. NTEC1 and non-NTEC were not frequently antibiotic resistant. The most frequent resistant were to ampicillin, trimethoprim/sulfonamides and neomycin.
[en] The purpose of this study was to characterize 25 necrotoxigenic Escherichia coli type 1 (NTEC1), one necrotoxigenic E coli type 2 (NTEC2) and 16 non-necrotoxigenic E coli isolated, between 1979 and 1993, from feces, intestinal content or internal organs of dogs and cats using a uniform and standardized typing scheme, as previously described on NTEC1 and NTEC2 isolates from cattle, humans and pigs (Mainil et al, 1999). The results obtained by colony hybridization and PCR on the NTEC1 isolates were homogeneous: eighteen NTEC1 isolates harboured prs- and sfa/foc-related DNA sequences; five NTEC1 isolates harboured sfa/foc-related DNA sequences; one NTEC1 isolate harboured prs-related DNA sequences; and one was negative. The only NTEC2 isolate was also negative. Only a few NTEC1 isolates were positive for f17-, afa- or cdt-related DNA sequences. All NTEC1 and the NTEC2 isolates were haemolytic on blood agar and were resistant to the bactericidal activity of the serum (one exception). By contrast a very few produced an aerobactin or a colicin. The strains belonged to various serogroups and many could not be identified with the immunsera used. On the other hand all but one NTEC1 isolates belonged to biotype 9. The most typical profile identifed for NTEC1 from dogs and cats was: CNF1+Prs+S+Hly+Bio9+. The results obtained on the non-necrotoxigenic E. coli isolates were more heterogeneous: two of them harboured pap/prs- and sfa/foc-related DNA sequences; ten harboured pap/prs-related DNA sequences; and four, other DNA sequences. The results of the phenotypic assays were more heterogeneous also. NTEC1 strains were isolated from essentially young puppies and kitties (< 3 months for 16 out of 22), with sometimes older animals (up to 12 years). The digestive pathologies were non haemorrhagic diarrhea/enteritis essentially. When haemorrhagic diarrhea/enteritis were observed, other infectious agents were present or strongly suspected (parvovirus, Salmonella). Extraintestinal pathologies were septicaemic or systemic colibacillosis, with one case of necro-haemorrhagic metritis. NTEC1 and non-NTEC were not frequently antibiotic resistant. The most frequent resistant were to ampicillin, trimethoprim/sulfonamides and neomycin.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Mainil, Jacques ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Wilbaux, Myriam
Jacquemin, Etienne
Oswald, Eric
Imberechts, Hein
Van Bost, Sigrid
Language :
French
Title :
Les souches pathogènes d'Escherichia coli chez les chiens et les chats: III) Données bactériologiques et cliniques sur les souches nécrotoxinogènes et sur celles positives pour des adhésines
Publication date :
2001
Journal title :
Annales de Médecine Vétérinaire
ISSN :
0003-4118
eISSN :
1781-3875
Publisher :
Annales Medecine Veterinaire, Liege, Belgium
Volume :
145
Issue :
6
Pages :
343-354
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 21 November 2012

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