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Measurement of serum albumin by protein electrophoresis and bromocresol green methods in canine and equine patients
Ramery, Eve; Bureau, Fabrice
2012In Veterinary Clinical Pathology, 41 (4), p. 5
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Keywords :
Albumin; electrophoresis; bromocresol green
Abstract :
[en] Background: Bromocresol green method (BCG) is routinely used in veterinary laboratories to measure albumin. A good agreement between BCG and electrophoresis (SPE) in healthy domestic animals is reported. However, in human medicine, at low albumin and high globulin concentrations, BCG also binds to α- and β-globulins fractions. Surprisingly, there is no recent literature evaluating the BCG method in diseased domestic animals. Objective: The purpose of the present study was therefore to compare the serum albumin values obtained by SPE and BCG in canine and equine patients. Methods: Albumin measured by BCG and SPE was compared in the serum from 30 canine and 30 equine patients. Results: Good correlation was found (Spearman´s rho rs = 0.91 in dogs and 0.84 in horses). However, Altman and Bland analysis of results showed a positive bias of BCG with SPE (3.41 g/l in horses and 0.41 g/l in dogs) that increased when only hypoalbuminemic patients were considered (4.17 g/l in horses and 1.31 g/l in dogs). Conclusion: Results confirm that, despite good correlation, BCG method tends to overestimate albumin, especially in hypoalbuminemic patients. This is particularly evident in horses.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Ramery, Eve ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Biochimie
Bureau, Fabrice ;  Université de Liège - ULiège > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire
Language :
English
Title :
Measurement of serum albumin by protein electrophoresis and bromocresol green methods in canine and equine patients
Publication date :
December 2012
Event name :
15th Biennial Congress of the International Society for Animal Clinical Pathology & 14th Conference of the European Society of Veterinary Clinical Pathology
Event organizer :
ISACP & ESVCP
Event place :
Ljubjana, Slovenia
Event date :
du 02 au 09 Juillet 2012
Audience :
International
Journal title :
Veterinary Clinical Pathology
ISSN :
0275-6382
Publisher :
American Society for Veterinary Clinical Pathology, Baton Rouge, United States - Louisiana
Volume :
41
Issue :
4
Pages :
E5
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 November 2012

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