[en] Endosulfan is an organochlorine pesticide that was recently labeled as a persistent organic pollutant, but it is still widely employed, particularly in developing countries. The goal of this study is to evaluate the acute (LC50) and chronic effects (developmental and behavioural traits) of this insecticide on Rana dalmatina tadpoles after exposure to ecologically relevant concentrations (0.005, 0.01, and 0.05 mg/L) by applying video-tracking techniques to evaluate the quantitative effect of endosulfan on amphibian behavioural patterns. The 96 h LC50 value was 0.074 mg endosulfan/L. Tadpoles chronically exposed to 0.01 and 0.05 mg endosulfan/L underwent high mortality rate, decreased larval growth, delayed development, and increased incidence of malformations, and they did not reach metamorphosis by the end of the experiment. Moreover, tadpoles exposed to these concentrations exhibited several abnormalities in swimming patterns, such as shorter distance moved, swirling, resting, and unusual use of space. The exposure to 0.005 mg endosulfan/L did not cause any significant effects on behaviour, larval growth, or development, but we observed a significant decrease in both survival and time to metamorphosis. We showed that developmental abnormalities are dose-dependent and that the pesticide effects could differ depending on the endosulfan concentration and the species tested. We also validated the hypothesis that behavioural analysis, along with the use of new analytical methods, could be a useful tool in amphibian ecotoxicological studies.
Research center :
AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège
Lavorato, Manuela; Université de Liège - ULiège & University of Calabria > Unité de Biologie du Comportement & Department of Ecology
Bernabo, Ilaria; University of Calabria
Crescente, Antonio; University of Calabria
Denoël, Mathieu ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Biologie du comportement - Ethologie et psychologie animale
Tripepi, Sandro; University of Calabria
Brunelli, Elvira; University of Calabria
Language :
English
Title :
Endosulfan effects on Rana dalmatina tadpoles: Quantitative developmental and behavioural analysis
Publication date :
2013
Journal title :
Archives of Environmental Contamination and Toxicology
ISSN :
0090-4341
eISSN :
1432-0703
Publisher :
Springer Science & Business Media B.V., New York, United States - New York
Volume :
64
Issue :
2
Pages :
253-262
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] FRFC - Fonds de la Recherche Fondamentale Collective [BE]
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