Reference : Development of a generic micellar electrokinetic chromatography method for the separatio...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/133854
Development of a generic micellar electrokinetic chromatography method for the separation of 15 antimalarial drugs as a tool to detect medicine counterfeiting
English
Lamalle, Caroline mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Marini Djang'Eing'A, Roland mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Debrus, Benjamin [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Lebrun, Pierre mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Crommen, Jacques [Université de Liège - ULg > Département de pharmacie > Département de pharmacie >]
Hubert, Philippe mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Servais, Anne-Catherine mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Fillet, Marianne mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
7-Aug-2012
Electrophoresis
Vch Publishers
33
Special Issue: Electromigration Techniques in Pharmaceutical Analysis
1669-1678
Yes (verified by ORBi)
International
0173-0835
Weinheim
Germany
[en] Antimalarial drugs ; Counterfeit medicines ; Design of experiments ; Micellar electrokinetic chromatography ; Quality control
[en] Since antimalarial drugs counterfeiting is dramatically present on the African market, the development of simple analytical methods for their quality control is of great importance. This work consists in the CE analysis of 15 antimalarials (artesunate, artemether, amodiaquine, chloroquine, piperaquine, primaquine, quinine, cinchonine, mefloquine, halofantrine, sulfadoxine, sulfalen, atovaquone, proguanil, and pyrimethamine). Since all these molecules cannot be ionized at the same pH, MEKC was preferred because it also allows separation of neutral compounds. Preliminary experiments were first carried out to select the most crucial factors affecting the antimalarials separation. Several conditions were tested and four parameters as well as their investigation domain were chosen: pH (5–10), SDS concentration (20–90 mM), ACN proportion (10–40%), and temperature (20–35°C). Then, the experimental design methodology was used and a central composite design was selected. Mathematical modeling of the migration times allowed the prediction of optimal conditions (29°C, pH 6.6, 29 mM SDS, 36% ACN) regarding analyte separation. The prediction at this optimum was verified experimentally and led to the separation of 13 compounds within 8 min. Finally, the method was successfully applied to the quality control of African antimalarial medicines for their qualitative and quantitative content.
Centre Interfacultaire de Recherche du Médicament
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS; Léon Fredericq Foundation
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Researchers ; Professionals ; Students ; General public
http://hdl.handle.net/2268/133854
10.1002/elps.201100621
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