[en] Cell transfection relies upon the delivery of genomic material within cells in order to express selected DNA sequences. Non-viral gene delivery systems are preferable over viral vectors for several reasons: biosafety, low immunogenicity, higher loading capacity, and easiness of production. Their major drawbacks actually are their limited efficiency in vivo compared to viruses, their cytotoxicity and the fact that they are rapidly cleared up from the bloodstream. The aim of our study was to better characterize the physico-chemical behavior of the polycations based on poly(2-(dimethylamino)ethyl methacrylate)-co-poly(ethyleneglycol) and to control the formulation step to produce the polyelectrolyte complexes.
Research center :
CEIB - Centre Interfacultaire des Biomatériaux - ULiège
Disciplines :
Microbiology Biotechnology
Author, co-author :
Grandfils, Christian ; Université de Liège - ULiège > Biochimie et physiologie générale
Emonds-Alt, J.
Language :
English
Title :
Optimization of poly(2-(dimethylamino)ethyl methacrylate)-co-poly(ethyleneglycol)/DNA complexes designed for cell transfection
Publication date :
December 2005
Journal title :
Minerva Biotecnologica
ISSN :
1120-4826
eISSN :
1827-160X
Publisher :
Edizioni Minerva Medica, Turin, Italy
Volume :
17
Issue :
4
Pages :
237-243
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Nouveaux polycations et vectorisation d’ADN dans les cellules de parois vasculaires” Convention Région Wallonne n° 14612, année 2001-2004 dans le cadre du concours Initiative 2000
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