Immunocytochemistry of the golden hamster pituitary during chronic administration of diethylstilbestrol: a quantitative analysis using laser scanning microscopy
Van Cauwenberge, A.; Nonclercq, D.; Laurent, G.et al.
2001 • In Histochemistry and Cell Biology, 115, p. 169-178
α-MSH; Cell proliferation; Estrogens; Pars intermedia; Prolactin
Abstract :
[en] The aim of this study was to examine by immunohistochemistry the morphologic changes affecting pituitary cell populations in male Syrian hamsters undergoing chronic exposure (3 days to 9 months) to diethylstilbestrol (DES). Cell proliferation in the hypophysis was monitored by the immunohistochemical demonstration of S-phase cells after pulse labeling with 5-bromo-2'-deoxyuridine. Cell proliferation analysis was combined with the identification of different cell populations by immunostaining with antisera raised against hypophyseal hormones. Sections processed for double-label immunofluorescence were examined by confocal microscopy. In the adenohypophysis, the relative surface occupied by gonadotrophs and thyrotrophs decreased rapidly during the first months of treatment while corticotroph and somatotroph populations remained unaffected. Accordingly, the incidence of S-phase cells in these four cell populations was lower than or similar to control values. In contrast, lactotrophs increased gradually during the first month of exposure to DES to reach a maximum value at 2-4 months. At the beginning, this increase was primarily due to hyperplasia but later on it also involved cellular hypertrophy. Somatomammotrophs did not seem to be involved in this model. In the pars intermedia, the labeling index of melanotrophs rose rapidly to reach values 5-6 times higher than controls. After 4 months, neoplasms originating from the pars intermedia were seen invading both the neuro- and the adenohypophysis. At the end of treatment, the pituitary was markedly enlarged resulting from the development of an adenoma of the pars intermedia
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Van Cauwenberge, A.; Université de Mons-Hainaut - UMH
Nonclercq, D.; Université de Mons-Hainaut - UMH
Laurent, G.; Université de Mons-Hainaut - UMH
Zanen, G.; Université de Mons-Hainaut - UMH
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Alexandre, H.; Université de Mons-Hainaut - UMH
Heuson-Stiennon, J. A.; umh
Toubeau, G.; Université de Mons-Hainaut - UMH
Language :
English
Title :
Immunocytochemistry of the golden hamster pituitary during chronic administration of diethylstilbestrol: a quantitative analysis using laser scanning microscopy
Publication date :
2001
Journal title :
Histochemistry and Cell Biology
ISSN :
0948-6143
eISSN :
1432-119X
Publisher :
Springer Science & Business Media B.V., New York, United States - New York
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