Article (Scientific journals)
A panel of biological tests reveals developmental effects of pharmaceutical pollutants on late stage zebrafish embryos.
Pruvot, Benoist; Quiroz O' Donova, Yobhana; Voncken, Audrey et al.
2012In Reproductive Toxicology
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Abstract :
[en] Standard toxicological assays using the zebrafish model system evaluate lethality and teratogenicity upon exposure during the first two days after fertilization. We tested the biological effects of several widely used drugs on zebrafish by acute treatment for 24hours starting at late embryonic stages, between 48 and 72hours post-fertilization. For 4 out of 6 compounds, we observed a higher sensitivity of late stage zebrafish embryos for general toxicity (lethality) compared to younger embryos. Morphological defects such as edema, body curvature, delayed growth, decreased heart rate and locomotion were observed for each of the compounds tested, often at sublethal concentrations. Gene expression studies on a set of four selected genes revealed a specific regulatory pattern for the different compounds tested. Our results allow us to compare various toxicological endpoints and may contribute to the design of a rational high throughput approach using the zebrafish model.
Research center :
AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège
Giga-Development and Stem Cells - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Environmental sciences & ecology
Author, co-author :
Pruvot, Benoist ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Quiroz O' Donova, Yobhana ;  Université de Liège - ULiège > SYST -- ACC -- AD ULg
Voncken, Audrey ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Jeanray, Nathalie ;  Université de Liège - ULiège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes et modélisation
PIOT, Amandine ;  Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie
Muller, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
A panel of biological tests reveals developmental effects of pharmaceutical pollutants on late stage zebrafish embryos.
Publication date :
2012
Journal title :
Reproductive Toxicology
ISSN :
0890-6238
eISSN :
1873-1708
Publisher :
Elsevier, Netherlands
Pages :
in press
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2012. Published by Elsevier Inc.
Available on ORBi :
since 26 October 2012

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