Reference : A panel of biological tests reveals developmental effects of pharmaceutical pollutant...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Environmental sciences & ecology
http://hdl.handle.net/2268/132933
A panel of biological tests reveals developmental effects of pharmaceutical pollutants on late stage zebrafish embryos.
English
Pruvot, Benoist [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Quiroz O' Donova, Yobhana mailto [Université de Liège - ULg > > > SYST -- ACC -- AD ULg]
Voncken, Audrey mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Jeanray, Nathalie mailto [Université de Liège - ULg > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes et modélisation >]
PIOT, Amandine [Centre Hospitalier Universitaire de Liège - CHU > > Chimie médicale >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > Département des sciences de la vie >]
Muller, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
2012
Reproductive Toxicology
in press
Yes (verified by ORBi)
International
0890-6238
[en] Standard toxicological assays using the zebrafish model system evaluate lethality and teratogenicity upon exposure during the first two days after fertilization. We tested the biological effects of several widely used drugs on zebrafish by acute treatment for 24hours starting at late embryonic stages, between 48 and 72hours post-fertilization. For 4 out of 6 compounds, we observed a higher sensitivity of late stage zebrafish embryos for general toxicity (lethality) compared to younger embryos. Morphological defects such as edema, body curvature, delayed growth, decreased heart rate and locomotion were observed for each of the compounds tested, often at sublethal concentrations. Gene expression studies on a set of four selected genes revealed a specific regulatory pattern for the different compounds tested. Our results allow us to compare various toxicological endpoints and may contribute to the design of a rational high throughput approach using the zebrafish model.
Applied and Fundamental FISH Research Center - AFFISH-RC ; Giga-Development and Stem Cells
Researchers ; Professionals
http://hdl.handle.net/2268/132933
Copyright (c) 2012. Published by Elsevier Inc.

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