Reference : A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Spe...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/132831
A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Wel
English
Mille, Caroline []
Debarnot, Dominique []
Zorzi, Willy mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie >]
Elmoualij, Benaïssa mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie >]
Quadrio, Isabelle []
Perret-Liaudet, Armand []
Coudreuse, Arnaud []
Legeay, Gilbert []
Poncin-Epaillard, Fabienne []
15-Oct-2012
Biosensors
Elsevier Science
2
4
433-447
Yes (verified by ORBi)
International
0265-928X
Barking
United Kingdom
[en] CJD ; Proteins ; tensioactif agents
[en] This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the background signal and lead to a low sensitivity and false positives. It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains. The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein. Beside the surface chemistry effect, these different results are associated with protein conformation.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/132831
http://www.mdpi.com/2079-6374/2/4/433

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