Reference : EVALUATION OF SV2Alox/Cre TRANSGENIC MOUSE USING [18F]UCB-H IN IN VITRO AUTORADIOGRAPHY
Scientific congresses and symposiums : Poster
Human health sciences : Radiology, nuclear medicine & imaging
Life sciences : Multidisciplinary, general & others
http://hdl.handle.net/2268/134403
EVALUATION OF SV2Alox/Cre TRANSGENIC MOUSE USING [18F]UCB-H IN IN VITRO AUTORADIOGRAPHY
English
Serrano Navacerrada, Maria Elisa mailto [Université de Liège > > Centre de recherches du cyclotron >]
Becker, Guillaume mailto [Université de Liège > > Centre de recherches du cyclotron >]
MENTEN, Catherine mailto [Centre Hospitalier Universitaire de Liège - CHU > > Génétique >]
Bahri, Mohamed Ali mailto [Université de Liège > > Centre de recherches du cyclotron >]
Warnier, Corentin mailto [Université de Liège > > Centre de recherches du cyclotron >]
Mievis, Frédéric mailto [Université de Liège > > Centre de recherches du cyclotron >]
Giacomelli, Fabrice mailto [Université de Liège > > Centre de recherches du cyclotron >]
Lemaire, Christian mailto [Université de Liège > > Centre de recherches du cyclotron >]
Salmon, Eric mailto [Université de Liège > Département des sciences cliniques > Neuroimagerie des troubles de la mémoire et révalid. cogn. >]
Luxen, André mailto [Université de Liège > Département de chimie (sciences) > Laboratoire de chimie organique de synthèse >]
Rogister, Bernard mailto [Université de Liège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine >]
Plenevaux, Alain mailto [Université de Liège > > Centre de recherches du cyclotron >]
4-Sep-2015
Poster
No
No
International
International Symposium "Aging and sleep: Perks for longevity?
04 septembre 2015
G Vandewalle, S Chellappa, P Maquet & Ph Peigneux
Liège
Belgium
[en] Epilepsy ; [18F]UCB-H ; hippocampus ; cre-lox technique ; floxed-mice ; SV2A ; in vitro autoradiography
[en] Background: SV2A is the most studied isoform of the Synaptic Vesicle 2 proteins, which are involved in the synaptic vesicle trafficking. Interestingly, the SV2A has been identify as the binding site for the antiepileptic drug levetiracetam, showing a close relation between the epilepsy, the dysregulation of the SV2A levels and the response to antiepileptic medications. SV2A floxed-mice were developed using a cre-lox technique, leading to a strong decrease of SV2A expression in the CA3 field of the hippocampus. We aim here to validate this model using [18F]UCB-H, a novel PET imaging radiotracer with a nanomolar affinity for human SV2A.
Methods: In vitro autoradiography were performed on SV2Alox/Cre+ transgenic mouse brain slices. SV2Alox/Cre- mouse was used as control. To obtain a structural reference, brain slices underwent eosin-haematoxylin staining. Images of both procedures were coregistered using π-PMOD software. Regions of interest (Dentate Gyrus, CA1, CA2 and CA3) were drawn according to a stereotaxic atlas of the mouse brain.
Results: Analyses showed significant differences in radiotracer binding (p<0.001) between SV2Alox/Cre+ mouse and SV2Alox/Cre- mouse highlighting an important reduction for the labelling density in Ammon's horn, particularly in CA1, compared to Dentate Gyrus where the diminution was less marked.
Conclusions: Here, we used the radiotracer [18F]UCB-H to probe the decreased expression of SV2A protein in the hippocampus of SV2Alox/Cre+ mouse versus SV2Alox/Cre- control mouse. Our results contribute to the validation of the model, and encourage us to proceed with further longitudinal and behavioural studies.
Centre de Recherches du Cyclotron - CRC
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
http://hdl.handle.net/2268/134403
also: http://hdl.handle.net/2268/132695

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